On July 20, 2016, Wei, Ping; Gao, Jia-Xin; Zheng, Gao-Wei; Wu, Hong; Zong, Min-Hua; Lou, Wen-Yong published an article.HPLC of Formula: 3976-69-0 The title of the article was Engineering of a novel carbonyl reductase with coenzyme regeneration in E. coli for efficient biosynthesis of enantiopure chiral alcohols. And the article contained the following:
The novel anti-Prelog stereospecific carbonyl reductase from Acetobacter sp. CCTCC M209061 was successfully expressed in E. coli combined with glucose dehydrogenase (GDH) to construct an efficient whole-cell biocatalyst with coenzyme NADH regeneration. The enzymic activity of GAcCR (AcCR with a GST tag) reached 304.9 U/g-dcw, even 9 folds higher than that of wild strain, and the activity of GDH for NADH regeneration recorded 46.0 U/mg-protein in the recombinant E. coli. As a whole-cell biocatalyst, the recombinant E. coli BL21(DE3)pLysS (pETDuet-gaccr-gdh) possessed a broad substrate spectrum for kinds of carbonyl compounds with encouraging yield and stereoselectivity. Besides, the asym. reduction of Et 4-chloroacetoacetate (COBE) to optically pure Et 4-chloro-3-hydroxybutyrate (CHBE) catalyzed by the whole-cell biocatalyst was systematically investigated. Under the optimal reaction conditions, the optical purity of CHBE was over 99% e.e. for (S)-enantiomer, and the initial rate and product yield reached 8.04 μmol/min and 99.4%, resp. Moreover, the space-time yield was almost 20 folds higher than that catalyzed by the wild strain. Therefore, a new, high efficiency biocatalyst for asym. reductions was constructed successfully, and the enantioselective reduction of prochiral compounds using the biocatalyst was a promising approach for obtaining enantiopure chiral alcs. The experimental process involved the reaction of (R)-Methyl 3-hydroxybutanoate(cas: 3976-69-0).HPLC of Formula: 3976-69-0
The Article related to carbonyl reductase biosynthesis enantiopure chiral alc, (s)-chbe, acetobacter sp, asymmetric reduction, chiral alcohols, gaccr, whole-cell biocatalysis, Fermentation and Bioindustrial Chemistry: Methods (Including Analysis) and other aspects.HPLC of Formula: 3976-69-0
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics