Tag: 300-400

Watanabe, Rei; Hu, Yaping; Iio, Keita; Yoneda, Kozo; Hattori, Atsunori; Arai, Atsushi; Kigoshi, Hideo; Kita, Masaki published an article in 2018, the title of the article was Specific protein-labeling and ligand-binding position analysis with amidopyrene probes as LDI MS tags.Product Details of 79642-50-5 And the article contains the following content:

To readily analyze the binding mode of protein-ligand interactions, we developed ligand-bound-type and ligand-dissociation-type probes having 6-amidopyrene (apy) as a detecting group. Matrix- and label-assisted laser desorption/ionization mass spectrometry (MALDI and LA-LDI MS) analyses and a covalent docking simulation using these probes precisely determined the binding position of the ligand biotin on the target protein avidin (RMSD = 0.786 and 0.332 Å). Our apy-probe-labeling method may be useful for determining the unknown ligand-binding sites of various target proteins. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Product Details of 79642-50-5

The Article related to protein labeling ligand interaction amidopyrene probe ldi mass spectrometry, Biochemical Methods: Synthesis and other aspects.Product Details of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Bian, Shudan; He, Jiajun; Schesing, Kevin B.; Braunschweig, Adam B. published an article in 2012, the title of the article was Polymer pen lithography (PPL)-induced site-specific click chemistry for the formation of functional glycan arrays.Electric Literature of 79642-50-5 And the article contains the following content:

PPL was combined successfully with the CuAAC to form functional mol. arrays over large (> cm2) areas and with feature diameters <1 μm. The problem of the differential transport rates of the reactive components through the aqueous meniscus was solved by embedding them within a PEG matrix. Covalent immobilization as a result of the CuAAC was demonstrated by fluorescence microscopy, and electrochem. anal. confirmed that the surface reactions produce features with high coverage d. Finally, the utility of this approach to glycomics was shown by creating an active array of α-mannose that could be recognized by ConA. In combination with previously reported multiplexing strategies, the authors believe that the approach reported herein could be employed widely to create arrays of biol. probes where the activity has a critical dependence on probe orientation. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Electric Literature of 79642-50-5

The Article related to polymer pen lithog site specific click chem glycan microarray, Biochemical Methods: Apparatus and other aspects.Electric Literature of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On December 31, 2016, Hsieh, Tsung-Han S.; Fudenberg, Geoffrey; Goloborodko, Anton; Rando, Oliver J. published an article.Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome. And the article contained the following:

We present Micro-C XL, an improved method for anal. of chromosome folding at mononucleosome resolution Using long crosslinkers and isolation of insoluble chromatin, Micro-C XL increases signal-to-noise ratio. Micro-C XL maps of budding and fission yeast genomes capture both short-range chromosome fiber features such as chromosomally interacting domains and higher order features such as centromere clustering. Micro-C XL provides a single assay to interrogate chromosome folding at length scales from the nucleosome to the full genome. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to chromosome folding nucleosome dna conformation micro c xl, Biochemical Genetics: Methods and other aspects.Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On February 21, 2020, Geng, Xueyun; Wang, Guirong; Guo, Zhongwu; Gu, Guofeng published an article.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Synthesis of the Oligosaccharides of Burkholderia pseudomallei and B. mallei Capsular Polysaccharide and Preliminary Immunological Studies of Their Protein Conjugates. And the article contained the following:

Efficient strategies were developed for the synthesis of 6-deoxy-D-manno-heptopyranose and its β-(1 â†?3)-linked oligomers as fragments of the common and major capsular polysaccharide, type I O-PS, of Burkholderia pseudomallei and Burkholderia mallei. The unusual heptose was synthesized from mannose, highlighted by the facile Wittig reaction and anti-Markovnikov hydroboration of the resultant olefin. The difficult β-mannosidic linkage in the oligosaccharides was achieved in high stereoselectivity by H-bond-mediated aglycon delivery. All of the oligosaccharides were conjugated with the carrier protein CRM197. Preliminary immunol. evaluations of the resultant glycoconjugates in mice verified their efficacy to elicit high titers of IgG antibodies and robust T-cell-dependent immune responses. It was also found that the trisaccharide conjugates provoked the strongest immune responses, worthy of further in-depth study for vaccine development. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to igg igm antibody oligosaccharide preparation vaccine glycosylation immunol protein, Carbohydrates: Oligosaccharides and other aspects.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On April 5, 2016, Killinger, Bryan A.; Moszczynska, Anna published an article.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Characterization of α-Synuclein Multimer Stoichiometry in Complex Biological Samples by Electrophoresis. And the article contained the following:

The aberrant aggregation of α-synuclein in the brain is a hallmark of Parkinson’s disease (PD). In vivo soluble α-synuclein occurs as a monomer and several multimers, the latter of which may be important for the biol. function of α-synuclein. Currently, there is a lack of reproducible methods to compare α-synuclein multimer abundance between complex biol. samples. Here we developed a method, termed “multimer-PAGE,” that combines in-gel chem. crosslinking with several common electrophoretic techniques to measure the stoichiometry of soluble α-synuclein multimers in brain tissue lysates. Results show that soluble α-synuclein from the rat brain exists as several high mol. weight species of approx. 56 kDa (αS56), 80 kDa (αS80), and 100 kDa (αS100) that comigrate with endogenous lipids, detergents, and/or micelles during blue native gel electrophoresis (BN-PAGE). Co-extraction of endogenous lipids with α-synuclein was essential for the detection of soluble α-synuclein multimers. Homogenization of brain tissue in small buffer volumes (>50 mg tissue per 1 mL buffer) increased relative lipid extraction and subsequently resulted in abundant soluble multimer detection via multimer-PAGE. α-Synuclein multimers captured by directly crosslinking soluble lysates resembled those observed following multimer-PAGE. The ratio of multimer (αS80) to monomer (αS17) increased linearly with protein input into multimer-PAGE, suggesting to some extent, multimers were also formed during electrophoresis. Overall, soluble α-synuclein maintains lipid interactions following tissue disruption and readily forms multimers when this lipid-protein complex is preserved. Once the multimer-PAGE technique was validated, relative stoichiometric comparisons could be conducted simultaneously between 14 biol. samples. Multimer-PAGE provides a simple inexpensive biochem. technique to study the mol. factors influencing α-synuclein multimerization. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to synuclein multimer assembly stoichiometry determination electrophoresis, Biochemical Methods: Electrical and other aspects.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On March 31, 2021, Nott, Alexi; Schlachetzki, Johannes C. M.; Fixsen, Bethany R.; Glass, Christopher K. published an article.Category: esters-buliding-blocks The title of the article was Nuclei isolation of multiple brain cell types for omics interrogation. And the article contained the following:

We present a nuclei isolation protocol for genomic and epigenomic interrogation of multiple cell type populations in the human and rodent brain. The nuclei isolation protocol allows cell type-specific profiling of neurons, microglia, oligodendrocytes, and astrocytes, being compatible with fresh and frozen samples obtained from either resected or postmortem brain tissue. This 2-day procedure consists of tissue homogenization with fixation, nuclei extraction, and antibody staining followed by fluorescence-activated nuclei sorting (FANS) and does not require specialized skillsets. Cell type-specific nuclei populations can be used for downstream omic-scale sequencing applications with an emphasis on epigenomic interrogation such as histone modifications, transcription factor binding, chromatin accessibility, and chromosome architecture. The nuclei isolation protocol enables translational examination of archived healthy and diseased brain specimens through utilization of existing medical biorepositories. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Category: esters-buliding-blocks

The Article related to multiple brain cell type nuclei isolation omic interrogation, Biochemical Methods: Biological and other aspects.Category: esters-buliding-blocks

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On September 8, 2017, Cai, Deqin; Xun, Chaochao; Tang, Feng; Tian, Xiaobo; Yang, Liyun; Ding, Kan; Li, Wenzhe; Le, Zhiping; Huang, Wei published an article.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Glycoconjugate probes containing a core-fucosylated N-glycan trisaccharide for fucose lectin identification and purification. And the article contained the following:

Glyco-PAMAM dendrimers and glyco-agarose beads bearing a core-fucosylated N-glycan trisaccharide GlcNAcβ1,4(Fucα1,6)GlcNAc or a nonfucose disaccharide GlcNAcβ1,4GlcNAc were successfully synthesized and characterized by monosaccharide anal. with HPAEC-PAD technique. These glycoconjugates as fucose lectin probes were applied in fucose-specific lectin detection and purification The model fucose lectin AAL indicated binding activity with the FITC-labeled PAMAM carrying core-fucose trisaccharide. An affinity chromatog. column stuffed with the agarose beads carrying core-fucosylated trisaccharide exhibited a good specificity in purification of AAL than nonfucose disaccharide agarose beads. These novel glycoconjugates bearing the precise and simplified core-fucose N-glycan structure provided a potential application for core-fucose-specific lectin discovery. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to glycoconjugate glycan trisaccharide fucose lectin, core-fucose, fucose-specific lectin, glyco-agarose, glyco-dendrimer, glycoconjugate, Biochemical Methods: Synthesis and other aspects.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On June 19, 2020, Roberts, Brett L.; Ma, Zhi-Xiong; Gao, Ang; Leisten, Eric D.; Yin, Dan; Xu, Wei; Tang, Weiping published an article.Application In Synthesis of tert-Butyl 2-(2-(2-(tosyloxy)ethoxy)ethoxy)acetate The title of the article was Two-Stage Strategy for Development of Proteolysis Targeting Chimeras and its Application for Estrogen Receptor Degraders. And the article contained the following:

Proteolysis targeting chimeras (PROTACs) have emerged as useful chem. probes and potential therapeutics by taking advantage of the ubiquitin-proteasome system to degrade intracellular disease-associated proteins. PROTACs are heterobifunctional mols. composed of a target protein ligand, E3 ubiquitin ligase ligand, and a linker between them. The generation of efficient PROTACs requires screening of many parameters, especially the lengths and types of the linkers. The authors report the proof-of-concept study using a two-stage strategy to facilitate the development of PROTACs against the estrogen receptor (ER). In stage one, a library of close to 100 PROTACs was synthesized by simply mixing a library of ERα ligands containing a hydrazide functional group at different positions with a preassembled library of E3 ligase ligands bearing different types and lengths of linkers with a terminal aldehyde group in a 1:1 ratio. Cell-based screening occurred without further purification, because the formation of the acylhydrazone linkage is highly efficient and produces water as the only byproduct. Compound A3 was the most potent ER degrader in two ER+ cell lines (DC50= ~10 nM, Dmax= â‰?95%). Stage two involved transformation to a more stable amide linker to generate a more drug-like mol. The new compound, AM-A3, showed comparable biol. activity (DC50 = 1.1 nM, Dmax = 98%) and induced potent antiproliferation (IC50= 13.2 nM, Imax= 69%) in MCF-7. This proof-of -concept study demonstrates that the two-stage strategy can significantly facilitate the development of PROTACs against ER without the tedious process of making large numbers of PROTACs one by one. It has the potential to be expanded to many other targets. The experimental process involved the reaction of tert-Butyl 2-(2-(2-(tosyloxy)ethoxy)ethoxy)acetate(cas: 882518-89-0).Application In Synthesis of tert-Butyl 2-(2-(2-(tosyloxy)ethoxy)ethoxy)acetate

The Article related to proteolysis targeting chimera estrogen receptor degrader, Pharmacology: Structure-Activity and other aspects.Application In Synthesis of tert-Butyl 2-(2-(2-(tosyloxy)ethoxy)ethoxy)acetate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On January 25, 2018, Crew, Andrew P.; Raina, Kanak; Dong, Hanqing; Qian, Yimin; Wang, Jing; Vigil, Dominico; Serebrenik, Yevgeniy V.; Hamman, Brian D.; Morgan, Alicia; Ferraro, Caterina; Siu, Kam; Neklesa, Taavi K.; Winkler, James D.; Coleman, Kevin G.; Crews, Craig M. published an article.SDS of cas: 882518-89-0 The title of the article was Identification and Characterization of Von Hippel-Lindau-Recruiting Proteolysis Targeting Chimeras (PROTACs) of TANK-Binding Kinase 1. And the article contained the following:

Proteolysis Targeting Chimeras (PROTACs) are bifunctional mols. that recruit an E3 ligase to a target protein to facilitate ubiquitination and subsequent degradation of that protein. While the field of targeted degraders is still relatively young, the potential for this modality to become a differentiated and therapeutic reality is strong, such that both academic and pharmaceutical institutions are now entering this interesting area of research. In this paper, the authors describe a broadly applicable process for identifying degrader hits based around the serine/threonine kinase TANK-binding kinase 1 (TBK1), and have generalized the key structural elements associated with degradation activities. Compound I is a potent hit (TBK1 DC50 = 12 nM, Dmax = 96%) with excellent selectivity against a related kinase IKKε, which was further used as a chem. tool to assess TBK1 as a target in mutant K-Ras cancer cells. The experimental process involved the reaction of tert-Butyl 2-(2-(2-(tosyloxy)ethoxy)ethoxy)acetate(cas: 882518-89-0).SDS of cas: 882518-89-0

The Article related to von hippel lindau protac tank binding kinase 1, Pharmacology: Structure-Activity and other aspects.SDS of cas: 882518-89-0

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Kim, Jee Young; Sung, Gun Yong; Park, Min published an article in 2020, the title of the article was Efficient portable urea biosensor based on urease immobilized membrane for monitoring of physiological fluids.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate And the article contains the following content:

Herein, a portable urea biosensor was developed for the real-time monitoring of the flow of physiol. fluids; this was achieved by using disuccinimidyl cross-linker-based urease immobilization. Urease was immobilized on a porous polytetrafluoroethylene (PTFE) solid support using different disuccinimidyl cross-linkers, namely disuccinimidyl glutarate (DSG), disuccinimidyl suberate (DSS) and bis-N-succinimidyl-(pentaethylene glycol) ester (BS(PEG)5). A urease activity test revealed that DSS exhibited the highest urease immobilizing efficiency, whereas FT-IR anal. confirmed that urease was immobilized on the PTFE membrane via DSS crosslinking. The membrane was inserted in a polydimethylsiloxane (PDMS) fluidic chamber that generated an electrochem. signal in the presence of a flowing fluid containing urea. Urea samples were allowed to flow into the urea biosensor (1.0 mL/min) and the signal was measured using chronoamperometry. The sensitivity of the DSS urea biosensor was the highest of all the trialed biosensors and was found to be superior to the more commonly used GA cross-linker. To simulate real-time monitoring in a human patient, flowing urea-spiked human serum was measured and the effective urease immobilization of the DSS urea biosensor was confirmed. The repeatability and interference of the urea biosensor were suitable for monitoring urea concentration typically found in human patients. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to body fluid urease urea biosensor, disuccinimidyl cross-linker, flow system, real-time monitoring, urea biosensor, urease immobilization, Biochemical Methods: Biological and other aspects.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics