A high-throughput HPLC method for simultaneous quantification of pyrethroid and pyriproxyfen in long-lasting insecticide-treated nets was written by Walker, Kyle J.;Williams, Christopher T.;Oladepo, Folasade O.;Lucas, John;Malone, David;Paine, Mark J. I.;Ismail, Hanafy M.. And the article was included in Scientific Reports in 2022.COA of Formula: C20H26O4 This article mentions the following:
Long-lasting insecticide-treated nets (LLINs) play a crucial role in preventing malaria transmission. LLINs should remain effective for at least three years, even after repeated washings. Currently, monitoring insecticides in LLINs is cumbersome, costly, and requires specialized equipment and hazardous solvents. Our aim was to develop a simple, high-throughput and low-resource method for measuring insecticides in LLINs. To extract insecticides, polyethylene-LLIN samples were heated at 85 °C for 45 min in a non-hazardous solvent mix containing dicyclohexylphthalate as an internal standard The extraction solvent was reduced from 50 to 5 mL using a 0.2 g sample, 90% smaller than the recommended sample size. By optimizing HPLC chromatog., we simultaneously detected pyrethroid and pyriproxyfen insecticides with high sensitivity in LLINâ²s extract The method can quantify levels â?0.0015% permethrin, 0.00045% alpha-cypermethrin and 0.00025% pyriproxyfen (weight/weight) in polyethylene, allowing for insecticide tracking before and after the use of LLINs. This method can be used to assess LLINs with 1% pyriproxyfen (pyriproxyfen-LLIN) or 2% permethrin (Olyzet Net), 1% pyriproxyfen and 2% permethrin (Olyzet Duo), or 0.55% pyriproxyfen and 0.55% alpha-cypermethrin (Royal Gaurd). One can run 120 samples (40 nets) simultaneously with high precision and accuracy, improving throughput and reducing labour, costs, and environmental impact. In the experiment, the researchers used many compounds, for example, Dicyclohexyl phthalate (cas: 84-61-7COA of Formula: C20H26O4).
Dicyclohexyl phthalate (cas: 84-61-7) belongs to esters. Esters are widespread in nature and are widely used in industry. In nature, fats are in general triesters derived from glycerol and fatty acids. Esters are responsible for the aroma of many fruits. Cyclic esters are called lactones, regardless of whether they are derived from an organic or inorganic acid. One example of an organic lactone is γ-valerolactone.COA of Formula: C20H26O4
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics