Dual-stimuli-responsive porous polymer enzyme reactor for tuning enzymolysis efficiency was written by Shen, Ji;Qiao, Juan;Zhang, Xinya;Qi, Li. And the article was included in Microchimica Acta in 2021.Application of 27249-90-7 This article mentions the following:
A strategy for preparing a dual-stimuli-responsive porous polymer membrane enzyme reactor (D-PPMER) is described, consisting of poly(styrene-maleic anhydride-N-isopropylacrylamide-acrylate-3′,3′-dimethyl-6-nitro-spiro[2H-1-benzopyran-2,2′-indoline]-1′-esterspiropyran ester) [P(S-M-N-SP)] and D-amino acid oxidase. Tunable control via “on/off” 365 nm UV light irradiation and temperature variation was used to change the membrane surface configuration and adjust the enzymolysis efficiency of the D-PPMER. A chiral capillary electrophoresis technique was developed for evaluation of the enzymic efficiency of D-PPMER with a Zn(II)-dipeptide complex as the chiral selector and D,L-serine as the substrate. Interestingly, the enzymic kinetic reaction rate of D-PPMER under UV irradiation at 36°C (9.2 x 10-2 mM·min-1) was 3.2-fold greater than that of the free enzyme (2.9 x 10-2 mM·min-1). This was because upon UV irradiation at high temperature, the P(SP) and P(N) moieties altered from a “stretched” to a “curled” state to encapsulate the enzyme in smaller cavities. The confinement effect of the cavities further improved the enzymic efficiency of the D-PPMER. This protocol highlights the outstanding potential of smart polymers, enables tunable control over the kinetic rates of stimuli-responsive enzyme reactors, and establishes a platform for adjusting enzymolysis efficiency using two different stimuli. In the experiment, the researchers used many compounds, for example, Benzyl benzodithioate (cas: 27249-90-7Application of 27249-90-7).
Benzyl benzodithioate (cas: 27249-90-7) belongs to esters. Esters typically have a pleasant smell; those of low molecular weight are commonly used as fragrances and are found in essential oils and pheromones. Acyl chlorides and acid anhydrides alcoholysis is another way to produce esters. Acyl chlorides and acid anhydrides react with alcohols to produce esters. Anydrous conditions are recommended since both acyl chlorides and acid anhydrides react with water.Application of 27249-90-7
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics