Combined use of analyte protectants and precolumn backflushing for a robust, high-throughput quantitative determination of aroma compounds in cigarette mainstream smoke by gas chromatography-tandem mass spectrometry was written by Guo, Qiong;Pan, Lining;Qin, Yaqiong;Xie, Fuwei;Wang, Xiaoyu;Zhao, Xiaodong;Chen, Li;Wang, Bing;Cai, Junlan;Liu, Huimin. And the article was included in Microchemical Journal in 2022.Electric Literature of C6H12O2 This article mentions the following:
Trace anal. of multi-targets in complex matrixes such as cigarette smoke has always been a great challenge for analysts. In this paper, a high-throughput qual. and quant. method was developed and validated to determine 559 aroma compounds in mainstream smoke using gas chromatog.-tandem mass spectrometry. Among four commonly used extraction solvents, dichloromethane showed obvious advantages in extracting the target aroma compounds with different polarity. An analyte protectant mixture consisting of 3-methoxy-1,2-propanediol, 1,2-octandiol, 1,2-nonanediol, and 1,2-tetradecanediol was first presented to compensate for the matrix effect, which was suitable for solvents of a wide polarity range such as hexane, dichloromethane, acetone, acetonitrile, and methanol. Moreover, the introduction of precolumn backflushing markedly improved the instrument robustness in analyzing complex cigarette smoke. The results showed that the system retained good stability after 30 days of injection. The proposed method performed well in terms of key validation parameters: accuracy (70.3-118.8% for 552 compounds), inter/intraday precision (0.1-10.7% and 0.2-18.2%), and sensitivity (limit of detection 0.12-102.54 ng/mL). Finally, this method was successfully applied to analyze 12 different com. cigarette brands. The proposed method has good application prospects based on its robustness and may be used as a potential tool to transfer the cigarette design from an experience-dependent mode to a digital design mode. This method also shows potential in assaying aroma compounds in essentials, food or plant-based substrates. In the experiment, the researchers used many compounds, for example, Methyl2-methylbutyrate (cas: 868-57-5Electric Literature of C6H12O2).
Methyl2-methylbutyrate (cas: 868-57-5) belongs to esters. Esters are widespread in nature and are widely used in industry. In nature, fats are in general triesters derived from glycerol and fatty acids. Esters are responsible for the aroma of many fruits, including apples, durians, pears, bananas, pineapples, and strawberries. Esters are more polar than ethers but less polar than alcohols. They participate in hydrogen bonds as hydrogen-bond acceptors, but cannot act as hydrogen-bond donors, unlike their parent alcohols. This ability to participate in hydrogen bonding confers some water-solubility.Electric Literature of C6H12O2
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics