Improvement of enantioselectivity and stability of Klebsiella oxytoca hydrolase immobilized on Eupergit C 250L was written by Wang, Pei-Yun;Tsai, Shau-Wei;Chen, Teh-Liang. And the article was included in Journal of Chemical Technology and Biotechnology in 2008.Recommanded Product: Ethyl 2-hydroxy-3-phenylpropanoate This article mentions the following:
A simple procedure was employed to covalently immobilize a Klebsiella oxytoca hydrolase (SNSM-87) onto epoxy-activated supports of Eupergit C 250L via multipoint covalent attachment. The resultant biocatalyst was explored for the hydrolytic resolution of a variety of (R,S)-2-hydroxycarboxylic acid Et esters. Results: With the hydrolytic resolution of (R,S)-Et mandelate in biphasic media as the model system, optimal conditions of 55°, pH 6 buffer and isooctane as the organic phase were selected for improving the enzyme stability (activity retained from 10% to 50% at 96 h) and enantioselectivity (VSVR-1 value enhanced from 44 to 319) in comparison to the performance of free enzyme. Moreover, the immobilized enzyme retained its activity and enantioselectivity after eight cycles of hydrolysis at 55°. When applying the resolution process to other (R,S)-2-hydroxycarboxylic acid Et esters, 2.4- to 4.0-fold enhancements of the enantioselectivity in general were obtainable. The enantioselectivity enhancement, good reusability and easy recovery after reaction indicate that the immobilized SNSM-87 may have the potential as an industrial biocatalyst for the preparation of optically pure 2-hydroxycarboxylic acids. In the experiment, the researchers used many compounds, for example, Ethyl 2-hydroxy-3-phenylpropanoate (cas: 15399-05-0Recommanded Product: Ethyl 2-hydroxy-3-phenylpropanoate).
Ethyl 2-hydroxy-3-phenylpropanoate (cas: 15399-05-0) belongs to esters. Esters perform as high-grade solvents for a broad array of plastics, plasticizers, resins, and lacquers, and are one of the largest classes of synthetic lubricants on the commercial market. Esterification is the general name for a chemical reaction in which two reactants (typically an alcohol and an acid) form an ester as the reaction product. Esters are common in organic chemistry and biological materials.Recommanded Product: Ethyl 2-hydroxy-3-phenylpropanoate
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics