He, Xi published the artcileConstruction and analysis of a food-grade Lactiplantibacillus plantarum esterase/lipase overexpression system, HPLC of Formula: 123-29-5, the main research area is food grade Lactiplantibacillus esterase lipase overexpression system.
Lactiplantibacillus plantarum (L. plantarum) is widely used in the food industry, especially in meat fermentation and dairy processing, due to its lipase and esterase activities. In this study, lp_1002 from L. plantarum WCFS1 was cloned into the food-grade gene expression vector pMG36n, with nisin used as a selective marker. Furthermore, pMG36n- lp_1002 was retransformed into L. plantarum WCFS1, and lp_1002 was overexpressed in the newly constructed L. plantarum WCFS1 strain. Moreover, recombinant Lp_1002 showed the characteristics of both lipase and esterase. The results from hydrolysis of p-nitrophenylphosphate (pNPP) and triglycerides showed that the hydrolysis activities of the recombinant Lp_1002 were 420% and 360% higher than that of the original strain, resp. In addition, the results of wine MLF fermentation suggest that the recombinant strain significantly reduced the contents of Et esters, acetate esters and other types of esters, which affected wine aroma during brewing. Therefore, the recombinant strain has great application potential in food fermentation
LWT–Food Science and Technology published new progress about Brewing. 123-29-5 belongs to class esters-buliding-blocks, name is Ethyl nonanoate, and the molecular formula is C11H22O2, HPLC of Formula: 123-29-5.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics