Bezerra, Rayanne M. published the artcileA new heterofunctional support for enzyme immobilization: PEI functionalized Fe3O4 MNPs activated with divinyl sulfone. Application in the immobilization of lipase from Thermomyces lanuginosus, Application In Synthesis of 140-11-4, the main research area is Thermomyces lanuginosus Fe3O4 lipase immobilization; Divinyl sulfone; Immobilization; Lipase from Thermomyces lanuginosus; Multipoint covalent attachment; Superparamagnetic nanoparticles.
Lipase from Thermomyces lanuginosus(TLL) was immobilized onto a novel heterofunctional support, divinyl sulfone (DVS) superparamagnetic nanoparticles (SPMNs) functionalized with polyethyleneimine (PEI). Particle size and zeta potential measurements, elemental anal., X-ray powder diffraction, magnetic measurements, and IR spectroscopy anal. were used to characterize the TLL preparations At pH 10, it was possible to achieve 100% of immobilization yield in 1 h. The immobilization pH gives TLL preparations with different stabilities; indeed the TLL preparation immobilized at pH 5.0 was the most stable during the thermal inactivation at all pH values. For the hydrolysis of racemic Me mandelate, the nanobiocatalysts immobilized at pH 5.0 and blocked with ethylenediamine (EDA) and ethanolamine (ETA) obtained good enantioselectivities (68% and 72%, resp.) with high catalytic activities in the reaction medium at pH 7.0. The operational stability of the systems was evaluated in the esterification reaction of benzyl alc., obtaining up to 61% conversion after the seventh reaction cycle. These results show that SPMN@PEI-DVS support is a robust strategy for the easy and rapid recovery of the nanobiocatalyst by applying a magnetic field, showing great potential for industrial applications.
Enzyme and Microbial Technology published new progress about Optimization. 140-11-4 belongs to class esters-buliding-blocks, name is Benzyl acetate, and the molecular formula is C9H10O2, Application In Synthesis of 140-11-4.
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