Tsakanova, Gohar published the artcileTwo-photon imaging of oxidative stress in living erythrocytes as a measure for human aging, SDS of cas: 2044-85-1, the main research area is oxidative stress erythrocyte two photon laser light microscopy.
According to the “”oxidative stress theory”” of aging, this process is accompanied by a progressive and irreversible accumulation of oxidative damage caused by reactive oxygen species (ROS). This, in turn, has a deleterious impact on mol. mechanisms in aging thereby altering the physiol. function of the organism, increasing the risk of different aging-related diseases, as well as impacting the life span. The aim of the current study was to investigate oxidative stress in living red blood cells (RBCs) in human aging as an oxidative stress-related pathol. condition. Two-photon laser scanning and light microscopy techniques were applied to analyze the oxidative stress in RBCs and the cell viability. Spectrophotometric analyzes were performed to determine the percentage of RBC hemolysis, activities of superoxide dismutase and catalase in RBCs, as well as the ferroxidase activities of ceruloplasmin in blood plasma samples. The studies included three human aging groups, young, middle-aged, and elderly. According to the results, the two-photon fluorescence of carboxy-DCFDA, indicating the intensity of oxidative stress, significantly increase in RBCs by the increase of age (P < 0.05), and these intensities are in statistically significant pos. correlation with age (P < 0.001) and a strong neg. correlation (P < 0.05) with the activity of catalase in RBCs and ferroxidase activity of ceruloplasmin in plasma. In conclusion, two-photon fluorescent imaging of oxidative stress in human living RBCs is a valuable and accurate method for the determination of aging processes in humans and can be suggested as a novel indicator for human aging processes in individual aging. Biomedical Optics Express published new progress about Blood plasma. 2044-85-1 belongs to class esters-buliding-blocks, name is 2',7'-Dichloro-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate, and the molecular formula is C24H14Cl2O7, SDS of cas: 2044-85-1.
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