Mohd. Hussin, Fathin Najihah Nor published the artcileTaguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies, COA of Formula: C7H12O3, the main research area is Candida rugosa lipase immobilization nanocellulose; Alginate; Candida rugosa lipase; Enzyme immobilization; Montmorillonite; Nanocellulose; Taguchi optimization.
Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped. This study aimed to statistically optimize the covalent immobilization of Candida rugosa lipase (CRL) onto a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT). The coarser topol. and the presence of characteristic spherical globules in the field emission scanning electron micrographs and at. force micrographs, resp., supported the existence of CRL on ALG/NC/MMT. In addition, amide peaks at 3478 and 1640 cm-1 in the fourier transform IR spectra affirmed that CRL was covalently bonded to ALG/NC/MMT. The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35°C, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89% of Et levulinate (EL), as proven by gas chromatog.-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and NMR (CAS-539-88-8) data. A higher optimal reaction temperature (50°C) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.
Enzyme and Microbial Technology published new progress about Diutina rugosa. 539-88-8 belongs to class esters-buliding-blocks, name is Ethyl 4-oxopentanoate, and the molecular formula is C7H12O3, COA of Formula: C7H12O3.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics