Marullo, Philippe published the artcileMetabolic, organoleptic and transcriptomic impact of Saccharomyces cerevisiae genes involved in the biosynthesis of linear and substituted esters, Application of Ethyl 3-hydroxybutanoate, the main research area is Saccharomyces metabolism organoleptic transcriptomic biosynthesis; MGL2; YJU3; histone acetylation; substituted ester metabolism; wine fermentation.
Esters constitute a broad family of volatile compounds impacting the organoleptic properties of many beverages, including wine and beer. They can be classified according to their chem. structure. Higher alc. acetates differ from fatty acid Et esters, whereas a third group, substituted Et esters, contributes to the fruitiness of red wines. Derived from yeast metabolism, the biosynthesis of higher alc. acetates and fatty acid Et esters has been widely investigated at the enzymic and genetic levels. As previously reported, two pairs of esterases, resp. encoded by the paralogue genes ATF1 and ATF2, and EEB1 and EHT1, are mostly involved in the biosynthesis of higher alc. acetates and fatty acid Et esters. These esterases have a moderate effect on the biosynthesis of substituted Et esters, which depend on mono-acyl lipases encoded by MGL2 and YJU3. The functional characterization of such genes helps to improve our understanding of substituted ester metabolism in the context of wine alc. fermentation In order to evaluate the overall sensorial impact of esters, we attempted to produce young red wines without esters by generating a multiple esterase-free strain (δatf1, δatf2, δeeb1, and δeht1). Surprisingly, it was not possible to obtain the deletion of MGL2 in the δatf1/δatf2/δeeb1/δeht1 background, highlighting unsuspected genetic incompatibilities between ATF1 and MGL2. A preliminary RNA-seq anal. depicted the overall effect of the δatf1/δatf2/δeeb1/δeht1 genotype that triggers the expression shift of 1124 genes involved in nitrogen and lipid metabolism, but also chromatin organization and histone acetylation. These findings reveal unsuspected regulatory roles of ester metabolism in genome expression for the first time.
International Journal of Molecular Sciences published new progress about Acetates Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 5405-41-4 belongs to class esters-buliding-blocks, name is Ethyl 3-hydroxybutanoate, and the molecular formula is C6H12O3, Application of Ethyl 3-hydroxybutanoate.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics