Miyazawa, Toshifumi published the artcileResolution of non-protein amino acids via the microbial protease-catalyzed enantioselective hydrolysis of their N-unprotected esters, Related Products of esters-buliding-blocks, the main research area is amino acid ester resolution microbial protease enantioselective hydrolysis kinetics.
In the Aspergillus oryzae protease-catalyzed ester hydrolysis, substitution of N-unprotected amino acid esters for the corresponding N-protected amino acid esters resulted in a large enhancement of the hydrolysis rate, while the enantioselectivity was deteriorated strikingly when the substrates employed were the conventional Me esters. This difficulty was overcome by employing esters bearing a longer alkyl chain such as the iso-Bu ester. Utilizing this ester, amino acids carrying an aromatic side chain were resolved with excellent enantioselectivities (E=50 to >200). With amino acids bearing an aliphatic side chain also, good results in terms of the hydrolysis rate and enantioselectivity were obtained by employing such an ester as the iso-Bu ester. Moreover, the enantioselectivity proved to be enhanced further by conducting the reaction at low temperature This procedure was applicable to the case where the enantioselectivity was not high enough even by the use of the iso-Bu ester.
Tetrahedron published new progress about Aspergillus oryzae. 10047-10-6 belongs to class esters-buliding-blocks, name is Methyl 2-aminopentanoate hydrochloride, and the molecular formula is C6H14ClNO2, Related Products of esters-buliding-blocks.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics