Gimeno, Isabel published the artcileNon-Invasive Identification of Sex in Cultured Bovine Embryos by UHPLC-MS/MS Metabolomics, HPLC of Formula: 627-93-0, the publication is Metabolomics (2022), 18(8), 53, database is CAplus and MEDLINE.
Different gene expression between male and female bovine embryos leads to metabolic differences. We used UHPLC-MS/MS to identify sex metabolite biomarkers in embryo culture medium (CM). Embryos were produced in vitro under highly variable conditions, i.e., fertilized with 7 bulls, two breeds, and cultured with BSA or BSA + serum until Day-6. On Day-6, embryos were cultured individually for 24 h. CM of Day-7 embryos (86 female and 81 male) was collected, and Day-6 and Day-7 embryonic stages recorded. A study by sample subsets with fixed factors (culture, bull breed, and Day-6 and Day-7 stages) tentatively identified 31 differentially accumulated metabolites through 182 subsets. Day-6 and Day-7 stage together affected 13 and 11 metabolites resp., while 19 metabolites were affected by one or another stage and/or day. Culture supplements and individual bull changed 19 and 15 metabolites, resp. Single bull exerted the highest influence (20 metabolites with the significantly highest p values). Lipid (93 subsets; 11 metabolites) and amino acid (55 subsets; 13 metabolites) were the most relevant classes for sex identification. Single biomarker led to inefficient sex diagnosis, while metabolite combinations accurately identified sex. Our study is a first in non-invasive sex identification in cattle by overcoming factors that induce metabolic variation.
Metabolomics published new progress about 627-93-0. 627-93-0 belongs to esters-buliding-blocks, auxiliary class Ploymers, name is Dimethyl adipate, and the molecular formula is C8H14O4, HPLC of Formula: 627-93-0.
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https://en.wikipedia.org/wiki/Ester,
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