Insights into the mode of action of 2-(4-methoxyphenyl)ethyl isothiocyanate on Aspergillus niger was written by Wu, Tian-Lin;Hu, Yong-Mei;Sun, Yu;Zhang, Zhi-Jun;Wu, Zheng-Rong;Zhao, Wen-Bin;Tang, Chen;Du, Sha-Sha;He, Ying-Hui;Ma, Yue;Yang, Cheng-Jie;Liu, Ying-Qian. And the article was included in Food Control in 2022.Safety of Isopropylisothiocyanate The following contents are mentioned in the article:
Isothiocyanates had attracted attention for many years due to their antimicrobial properties. In this study, the antifungal efficiency of eighteen isothiocyanate compounds were evaluated against six plant pathogenic fungi and two molds in vitro. Among them, 2-(4-methoxyphenyl)ethyl isothiocyanate displayed the highest antifungal activity against Aspergillus niger (EC50 = 4.19μg/mL). The further studies of its mode of action showed that it may damage the cell morphol. and membrane integrity in a dose dependent manner by morphol. observation, Pr iodide staining and the release of cellular contents. On the other hand, the decrease of related enzyme activities in tricarboxylic acid cycle suggested that 2-(4-methoxyphenyl)ethyl isothiocyanate may effected energy metabolism Besides, reactive oxygen species, the content of malondialdehyde, the total antioxidant capacity, the content of glutathione and the kinase of oxidative reactive were changed in fungi treated with 2-(4-methoxyphenyl)ethyl isothiocyanate, compared with those in untreated fungi, which all indicated that 2-(4-methoxyphenyl)ethyl isothiocyanate exhibited antifungal activity against A.niger by inducing oxidative stress. This study involved multiple reactions and reactants, such as Isopropylisothiocyanate (cas: 2253-73-8Safety of Isopropylisothiocyanate).
Isopropylisothiocyanate (cas: 2253-73-8) belongs to esters. Carboxylic acid esters of low molecular weight are colourless, volatile liquids with pleasant odours, slightly soluble in water. Esters are more polar than ethers but less polar than alcohols. They participate in hydrogen bonds as hydrogen-bond acceptors, but cannot act as hydrogen-bond donors, unlike their parent alcohols. This ability to participate in hydrogen bonding confers some water-solubility.Safety of Isopropylisothiocyanate
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics