Integrated analysis and knockdown of RAB23 indicate the role of RAB23 in gastric adenocarcinoma was written by Chen, Hui;Pan, Dun;Yang, Zhihuang;Li, Liangqing. And the article was included in Annals of Translational Medicine in 2019.Safety of Methyl nonadecanoate The following contents are mentioned in the article:
Background: The present study aimed to identify key differentially expressed genes (DEGs) and miRNAs (DEmiRNAs) in gastric adenocarcinoma. Methods: We performed integrated anal. to determine DEGs and DEmiRNAs of gastric adenocarcinoma based on the GEO database. A DEmiRNA-target interaction network was established. GO and KEGG pathway enrichment analyses were utilized. Then, MKN45 cells were transfected with shRNA-RAB23 to knock down the expression of RAB23. CCK-8, transwell and flow cytometry assays were utilized to measure the capacities for cell proliferation, migration and apoptosis, and the apoptosis-related gene and protein levels were measured by using polymerase chain reaction (PCR) and Western blot, resp. Colocalization anal. of Snc1 with the vesicular protein VAMP3 and the endoplasmic reticulum protein Calnexin was performed to assess the influence of RAB23 on vesicle transport. Finally, we performed metabolomic anal. by using gas chromatog. mass spectrometry (GC-MS). Results: We performed MMIA of gastric adenocarcinoma based on two miRNA datasets and two mRNA datasets. A total of 4,586 DEmRNAs and 30 DEmiRNAs were obtained. The DEmRNAs of gastric adenocarcinoma were significantly enriched in PI3K/Akt signaling. This study involved multiple reactions and reactants, such as Methyl nonadecanoate (cas: 1731-94-8Safety of Methyl nonadecanoate).
Methyl nonadecanoate (cas: 1731-94-8) belongs to esters. Esters are widespread in nature and are widely used in industry. In nature, fats are in general triesters derived from glycerol and fatty acids. Esters are responsible for the aroma of many fruits. Because of their lack of hydrogen-bond-donating ability, esters do not self-associate. Consequently, esters are more volatile than carboxylic acids of similar molecular weight.Safety of Methyl nonadecanoate
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics