Purification and properties of an esterase from Bacillus licheniformis and it′s application in synthesis of octyl acetate was written by Bhardwaj, Kamal K.;Dogra, Adarsh;Kapoor, Smita;Mehta, Akshita;Gupta, Reena. And the article was included in Open Microbiology Journal in 2020.HPLC of Formula: 112-14-1 The following contents are mentioned in the article:
Background: Esterase plays a major role in the degradation of natural materials, industrial pollutants and also provides an immense contribution to the ecofriendly approaches in various industrial applications. Objective: In the present study, extracellular esterase from bacterial isolate Bacillus licheniformis was purified, characterized and used in the synthesis of octyl acetate. Methods: Purification of esterase from Bacillus licheniformis was achieved using Sephadex G-75 column chromatog. Gas chromatog. was used to analyze the octyl acetate synthesis. Results: The enzyme was salted out using ammonium sulfate precipitation and 60-70% saturation gave maximum specific activity of the enzyme during precipitation A purification fold of 6.46 and yield of 9.69% was achieved when esterase from Bacillus licheniformis was purified using Sephadex G-75 column chromatog. This showed that the enzyme was purified to homogeneity and it was a monomer with mol. weight of 42 kDa. Biochem. characterization of the enzyme revealed that it had optimum temperature of 45°C in 0.1 M Tris-HCl buffer of pH 8.0. On optimizing different parameters, such as molar ratio of reactants, incubation time, temperature, and amount of protein, the % yield of octyl acetate was found to be 77.3%. Conclusion: In this work, simple method was used to purify esterase and the enzyme was further used in producing esters/products of com. value within a reasonably short period of 12 h with a maximum yield of 77.3%. This study involved multiple reactions and reactants, such as Octyl acetate (cas: 112-14-1HPLC of Formula: 112-14-1).
Octyl acetate (cas: 112-14-1) belongs to esters. Esters are widespread in nature and are widely used in industry. In nature, fats are in general triesters derived from glycerol and fatty acids. Esters are responsible for the aroma of many fruits. Esterification is the general name for a chemical reaction in which two reactants (typically an alcohol and an acid) form an ester as the reaction product. Esters are common in organic chemistry and biological materials.HPLC of Formula: 112-14-1
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics