Solid-phase microextraction-gas chromatography and ultra-high performance liquid chromatography applied to the characterization of lemon wax, a waste product from citrus industry was written by Costa, Rosaria;Albergamo, Ambrogina;Arrigo, Sara;Gentile, Fausto;Dugo, Giacomo. And the article was included in Journal of Chromatography A in 2019.Formula: C10H20O2 The following contents are mentioned in the article:
A novel application of SPME-GC and UHPLC is presented for the elucidation of an unexplored citrus byproduct, namely lemon wax. In view of the current environmental protection strategies, directed toward reuse and/or recovery of waste products, this study aimed to evaluate a possible utilization of a discarded fraction from citrus fruits processing. Headspace SPME coupled with GC-MS and GC-FID allowed determining a residual amount (162.25 mg kg-1) of lemon volatiles, by means of accurate calibration of 38 terpenoids through linear regression anal. (R2 = 0.99). SPME method development, was supported by statistical anal., consisting of exptl. design and multisample comparison, where parameters such as type of coating, temperature and time of fiber exposure, were manipulated towards optimization. The optimized method was validated, showing limits of quantification (LOQ) and of detection (LOD) in the ranges 0.24 – 2.65 ng g-1 and 0.11 – 0.87 ng g-1, resp.; recoveries ranged from 84.9% to 111.5%. After optimization and validation, the UHPLC-PDA method revealed the presence in lemon wax samples of six coumarins, the most abundant being bergamottin (964 mg kg-1), followed, in decreasing amount, by citropten, 8-geranyloxypsoralen, biacangelicin, oxypeucedanin, 5-geranyloxy-7-methoxycoumarin; each analyte was calibrated by external standard methodol. Sensitivity (LOD, 31 – 112 ng g-1; LOQ, 102-370 ng· g-1), accuracy (recovery, 81-94%), and precision (Retention time RSD, 0.03 – 0.09%; peak area RSD, 1.03-2.92%), were also measured. This study involved multiple reactions and reactants, such as Octyl acetate (cas: 112-14-1Formula: C10H20O2).
Octyl acetate (cas: 112-14-1) belongs to esters. Esters are widespread in nature and are widely used in industry. In nature, fats are in general triesters derived from glycerol and fatty acids. Esters are responsible for the aroma of many fruits. Liquid esters of low volatility serve as softening agents for resins and plastics. Esters also include many industrially important polymers. Polymethyl methacrylate is a glass substitute sold under the names Lucite and Plexiglas; polyethylene terephthalate is used as a film (Mylar) and as textile fibres sold as Terylene, Fortrel, and Dacron.Formula: C10H20O2
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics