In 2022,Shimizu, Kohei; Suzuki, Hitomi; Uemura, Takuya; Nozawa, Akira; Desaki, Yoshitake; Hoshino, Ryosuke; Yoshida, Ayako; Abe, Hiroshi; Nishiyama, Makoto; Nishiyama, Chiharu; Sawasaki, Tatsuya; Arimura, Gen-ichiro published an article in Plant Journal. The title of the article was 《Immune gene activation by NPR and TGA transcriptional regulators in the model monocot Brachypodium distachyon》.SDS of cas: 119-36-8 The author mentioned the following in the article:
The nonexpressor of pathogenesis-related (NPR) gene family is well known to play a crucial role in transactivation of TGA transcription factors for salicylic acid (SA)-responsive genes, including pathogenesis-related protein 1 (PR1), during plants immune response after pathogen attack in the model dicot Arabidopsis thaliana. However, little is known about NPR gene functions in monocots. We therefore explored the functions of NPRs in SA signaling in the model monocot Brachypodium distachyon. BdNPR1 and BdNPR2/3 share structural similarities with A. thaliana AtNPR1/2 and AtNPR3/4 subfamilies, resp. The transcript level of BdNPR2 but not BdNPR1/3 appeared to be pos. regulated in leaves in response to Me salicylate. Reporter assays in protoplasts showed that BdNPR2 pos. regulated BdTGA1-mediated activation of PR1. This transactivation occurred in an SA-dependent manner through SA binding at Arg468 of BdNPR2. In contrast, BdNPR1 functioned as a suppressor of BdNPR2/BdTGA1-mediated transcription of PR1. Collectively, our findings reveal that the TGA-promoted transcription of SA-inducible PR1 is orchestrated by the activator BdNPR2 and the repressor BdNPR1, which function competitively in B. distachyon. In the experiment, the researchers used Methyl Salicylate(cas: 119-36-8SDS of cas: 119-36-8)
Methyl Salicylate(cas: 119-36-8) has been used: as a component of clarifying solution for treating Mongolian gerbil cochlea intact for immunofluorescence analysis, as a plant elicitor to test its effect on reducing the whitefly population from tomato plants.SDS of cas: 119-36-8
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