On March 31, 2019, Chen, Kai; Huang, Xiongyi; Zhang, Shuo-Qing; Zhou, Andrew Z.; Kan, S. B. Jennifer; Hong, Xin; Arnold, Frances H. published an article.SDS of cas: 517-23-7 The title of the article was Engineered Cytochrome c-Catalyzed Lactone-Carbene B-H Insertion. And the article contained the following:
Previous work has demonstrated that variants of a heme protein, Rhodothermus marinus cytochrome c(Rma cyt c), catalyze abiol. carbene boron-hydrogen (B-H) bond insertion with high efficiency and selectivity. Here authors investigated this carbon-boron bond-forming chem. with cyclic, lactone-based carbenes. Using directed evolution, they obtained a Rma cyt c variant BORLAC that shows high selectivity and efficiency for B-H insertion of 5- and 6-membered lactone carbenes (up to 24,500 total turnovers and 97.1:2.9 enantiomeric ratio). The enzyme shows low activity with a 7-membered lactone carbene. Computational studies revealed a highly twisted geometry of the 7-membered lactone carbene intermediate relative to 5- and 6-membered ones. Directed evolution of cytochrome c together with computational characterization of key iron-carbene intermediates has allowed us to expand the scope of enzymic carbene B-H insertion to produce new lactone-based organoborons. The experimental process involved the reaction of 3-Acetyldihydrofuran-2(3H)-one(cas: 517-23-7).SDS of cas: 517-23-7
The Article related to cytochrome c catalyzed lactone carbene boron hydrogen insertion, mol structure calculation hemeprotein biocatalyst, biocatalyst, carbene, cytochrome c, directed evolution, lactones, organoboron and other aspects.SDS of cas: 517-23-7
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