Wood, David K.; Weingeist, David M.; Bhatia, Sangeeta N.; Engelward, Bevin P. published the artcile< Single cell trapping and DNA damage analysis using microwell arrays>, Safety of (9Z,12Z)-Methyl octadeca-9,12-dienoate, the main research area is single cell trapping DNA damage microwell array.
With a direct link to cancer, aging, and heritable diseases as well as a critical role in cancer treatment, the importance of DNA damage is well-established. The intense interest in DNA damage in applications ranging from epidemiol. to drug development drives an urgent need for robust, high throughput, and inexpensive tools for objective, quant. DNA damage anal. We have developed a simple method for high throughput DNA damage measurements that provides information on multiple lesions and pathways. Our method utilizes single cells captured by gravity into a microwell array with DNA damage revealed morphol. by gel electrophoresis. Spatial encoding enables simultaneous assays of multiple exptl. conditions performed in parallel with fully automated anal. This method also enables novel functionalities, including multiplexed labeling for parallel single cell assays, as well as DNA damage measurement in cell aggregates. We have also developed 24- and 96-well versions, which are applicable to high throughput screening. Using this platform, we have quantified DNA repair capacities of individuals with different genetic backgrounds, and compared the efficacy of potential cancer chemotherapeutics as inhibitors of a critical DNA repair enzyme, human AP endonuclease. This platform enables high throughput assessment of multiple DNA repair pathways and subpathways in parallel, thus enabling new strategies for drug discovery, genotoxicity testing, and environmental health.
Proceedings of the National Academy of Sciences of the United States of America published new progress about Computer program (comet anal. pipeline). 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Safety of (9Z,12Z)-Methyl octadeca-9,12-dienoate.
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