Chernov, Alexandr N.; Tsapieva, Anna; Alaverdian, Diana A.; Filatenkova, Tatiana A.; Galimova, Elvira S.; Suvorova, Mariia; Shamova, Olga V.; Suvorov, Alexander N. published the artcile< In Vitro Evaluation of the Cytotoxic Effect of Streptococcus pyogenes Strains, Protegrin PG-1, Cathelicidin LL-37, Nerve Growth Factor and Chemotherapy on the C6 Glioma Cell Line>, Application In Synthesis of 112-63-0, the main research area is anticancer PG1 LL37 NGF chemotherapy cytotoxicity Streptococcus glioma; LL-37; MTT assay; NGF; PG-1; Streptococcus pyogenes; cathelicidin family peptides; chemotherapy; cytotoxicity; glioma C6; human normal fibroblasts; real-time xCELLigence system; trypan blue assay.
Brain cancer treatment, where glioblastoma represents up to 50% of all CNS malignancies, is one of the most challenging calls for neurooncologists. The major driver of this study was a search for new approaches for the treatment of glioblastoma. We tested live S. pyogenes, cathelicidin family peptides and NGF, assessing the oncolytic activity of these compounds as monotherapy or in combination with chemotherapeutics. For cytotoxicity evaluation, we used the MTT assay, trypan blue assay and the xCELLigence system. To evaluate the safety of the studied therapeutic approaches, we performed experiments on normal human fibroblasts. Streptococci and peptides demonstrated high antitumor efficiency against glioma C6 cells in all assays applied, surpassing the effect of chemotherapeutics (doxorubicin, carboplatin, cisplatin, etoposide). A real-time cytotoxicity anal. showed that the cell viability index dropped to 21% 2-5 h after S. pyogenes strain exposure. It was shown that LL-37, PG-1 and NGF also exhibited strong antitumor effects on C6 glioma cells when applied at less than 10-4 M. Synergistic effects for combinations of PG-1 with carboplatin and LL-37 with etoposide were shown. Combinations of S. pyogenes strain #7 with NGF or LL-37 demonstrated a cytotoxic effect (56.7% and 57.3%, accordingly) on C6 glioma cells after 3 h of exposure.
Molecules published new progress about Antitumor agents. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Application In Synthesis of 112-63-0.
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