Kim, Jae-Hyun; Kim, Minsun; Hong, Sooyeon; Kim, Eun-Young; Lee, Hyangsook; Jung, Hyuk-Sang; Sohn, Youngjoo published the artcile< Albiflorin promotes osteoblast differentiation and healing of rat femoral fractures through enhancing BMP-2/Smad and Wnt/β-catenin signaling>, HPLC of Formula: 112-63-0, the main research area is Paeonia lactiflora albiflorin catenin osteoblast differentiation osteogenesis; Paeonia lactiflora; Wnt; albiflorin; bone morphogenetic protein 2; femoral fracture; osteoblast; runt-related transcription factor 2.
Fracture healing is related to osteogenic differentiation and mineralization. Recently, due to the unwanted side effects and clin. limitations of existing treatments, various natural productbased chem. studies have been actively conducted. Albiflorin is a major ingredient in Paeonia lactiflora, and this study investigated its ability to promote osteogenic differentiation and fracture healing. To demonstrate the effects of albiflorin on osteoblast differentiation and calcified nodules, alizarin red S staining and von Kossa staining were used in MC3T3-E1 cells. In addition, BMP-2/Smad and Wnt/β-catenin mechanisms known as osteoblast differentiation mechanisms were analyzed through RT-PCR and western blot. To investigate the effects of albiflorin on fracture healing, fractures were induced using a chainsaw in the femur of Sprague Dawley rats, and then albiflorin was i.p. administered. After 1, 2, and 3 wk, bone microstructure was analyzed using micro-CT. In addition, histol. anal. was performed by staining the fractured tissue, and the expression of osteogenic markers in serum was measured. The results demonstrated that albiflorin promoted osteoblastogenesis and the expression of RUNX2 by activating BMP-2/Smad and Wnt/β-catenin signaling in MC3T3-E1 cells. In addition, albiflorin upregulated the expression of various osteogenic genes, such as alk. phosphatase, OCN, bone sialoprotein, OPN, and OSN. In the femur fracture model, micro-CT anal. showed that albiflorin played a pos. role in the formation of callus in the early stage of fracture recovery, and histol. examination proved to induce the expression of osteogenic genes in femur tissue. In addition, the expression of bone-related genes in serum was also increased. This suggests that albiflorin promotes osteogenesis, bone calcification and bone formation, thereby promoting the healing of fractures in rats.
Frontiers in Pharmacology published new progress about Biomineralization. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, HPLC of Formula: 112-63-0.
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