Bonifacio, Maria Addolorata; Genchi, Chiara; Lagioia, Antonella; Talamo, Vincenza; Volpe, Anna; Mariggio, Maria Addolorata published the artcile< Analytical Assessment of the Vela Diagnostics NGS Assay for HIV Genotyping and Resistance Testing: The Apulian Experience>, Name: (9Z,12Z)-Methyl octadeca-9,12-dienoate, the main research area is diagnosis next generation sequencing HIV genotyping resistance; Sanger sequencing (SS); Vela Diagnostics; genotyping; human immunodeficiency virus (HIV); integrase strand transfer inhibitors (INSTIs); next-generation sequencing (NGS); non-nucleoside reverse transcriptase inhibitors (NNRTIs); nucleoside reverse transcriptase inhibitors (NRTIs); protease inhibitors (PIs); resistance-associated mutations (RAM).
Drug-resistance monitoring is one of the hardest challenges in HIV management. Next-generation sequencing (NGS) technologies speed up the detection of drug resistance, allowing the adjustment of antiretroviral therapy and enhancing the quality of life of people living with HIV. Recently, the NGS Sentosa SQ HIV Genotyping Assay (Vela Diagnostics) received approval for in vitro diagnostics use. This work is the first Italian evaluation of the performance of the Vela Diagnostics NGS platform, assessed with 420 HIV-1 clin. samples. A comparison with Sanger sequencing performance is also reported, highlighting the advantages and disadvantages of the Sentosa NGS assay. The precision of the technol. was studied with reference specimens, while intra- and inter-assay reproducibility were evaluated for selected clin. samples. Vela Diagnostics’ NGS assay reached an 87% success rate through 30 runs of anal. in a real-world clin. context. The concordance with Sanger sequencing outcomes was equal to 97.2%. Several detected mismatches were due to NGS’s superior sensitivity to low-frequency variants. A high accuracy was observed in testing reference samples. Repeatability and reproducibility assays highlighted the good performance of the NGS platform. Beyond a few tech. issues that call for further optimization, the key improvement will be a better balance between costs and processing speed. Once these issues have been solved, the Sentosa SQ HIV Genotyping Assay will be the way forward for HIV resistance testing.
International Journal of Molecular Sciences published new progress about Antiviral agents. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Name: (9Z,12Z)-Methyl octadeca-9,12-dienoate.
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