《The conjugation of rhodamine B enables carrier-free mitochondrial delivery of functional proteins》 was published in Organic & Biomolecular Chemistry in 2020. These research results belong to Shi, Jiayuan; Zhao, Dan; Li, Xiang; Ding, Feng; Tang, Xuemei; Liu, Nian; Huang, Hua; Liu, Changlin. Quality Control of N-tert-Butoxycarbonylhydroxylamine The article mentions the following:
The development of protein-based therapeutics faces many challenges, for example, carrier-dependence, safety concerns, endocytosis-dependence, and uncertain in vivo therapeutic outcomes. Small mols. are rarely used for intracellular organelle-targeting and disease tissue-specific carrier-independent delivery of therapeutic proteins. Here, the authors report that rhodamine B, after modification with proteins, is able to guide carrier-free delivery into mitochondria and tissue-dependent distributions of functional proteins through organic cation transporters (OCTs). The enrichment of the modified catalase in the cancer tissue efficiently suppresses xenograft human lung tumor in mice. This carrier-free delivery platform of proteins may emerge as a simple yet powerful approach for cancer treatment. The experimental part of the paper was very detailed, including the reaction process of N-tert-Butoxycarbonylhydroxylamine(cas: 36016-38-3Quality Control of N-tert-Butoxycarbonylhydroxylamine)
N-tert-Butoxycarbonylhydroxylamine(cas: 36016-38-3) belongs to anime. Halogenation, in which one or more hydrogen atoms of an amine is replaced by a halogen atom, occurs with chlorine, bromine, and iodine, as well as with some other reagents, notably hypochlorous acid (HClO). With primary amines the reaction proceeds in two stages, producing N-chloro- and N,N-dichloro-amines, RNHCl and RNCl2, respectively. With tertiary amines, an alkyl group may be displaced by a halogen.Quality Control of N-tert-Butoxycarbonylhydroxylamine
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