In 2022,Xu, Pingxi; Choo, Young-Moo; Leal, Walter S. published an article in Biochemical and Biophysical Research Communications. The title of the article was 《Odorant inhibition in mosquito olfaction mediated by inverse agonists》.Category: esters-buliding-blocks The author mentioned the following in the article:
The insect repellent Me salicylate elicits excitatory responses upon interaction with CquiOR32, an odorant receptor (OR) from the southern house mosquito, Culex quinquefasciatus. By contrast, eucalyptol binds to CquiOR32 to generate electrophysiol. and behavioral inhibitory responses. In an attempt to identify CquiOR32 variants displaying more robust inhibitory responses for more accurate current-voltage anal., we sequenced 31 CquiOR32 clones. In the Xenopus oocyte recording system, CquiOR32V2/CquiOrco-expressing oocytes yielded eucalyptol-elicited outward (inhibitory) currents relatively larger than Me salicylate-generated inward (excitatory) currents. Rescuing experiments showed that two of the amino acid substitutions in CquiOR32V2 located in a predicted transmembrane helix of the receptor are determinants of the outward/inward ratios. These findings, along with co-stimulus assays, suggest that odorant and inhibitor may bind to the same binding pocket. Current-voltage relationships obtained with standard perfusion buffer and those devoid of Na+ or Cl- indicated that both excitatory and inhibitory currents are mediated, at least in part, by cation. We then concluded that eucalyptol is an inverse agonist, which shifts the open closed equilibrium of the receptor toward the closed conformation, thus reducing the spontaneous activity. By contrast, the binding of Me salicylate shifts the equilibrium towards the open conformation and, consequently, leads to an increase in cation influx.Methyl Salicylate(cas: 119-36-8Category: esters-buliding-blocks) was used in this study.
Methyl Salicylate(cas: 119-36-8) has been used: as a component of clarifying solution for treating Mongolian gerbil cochlea intact for immunofluorescence analysis, as a plant elicitor to test its effect on reducing the whitefly population from tomato plants.Category: esters-buliding-blocks
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics