Zhang, Bolin; Liu, Ning; Kang, Kui; Zhang, Renbo; Hao, Meilin; Song, Peiyong; Wang, Qingrong; Xie, Yuxiao; Li, Chuntao published the artcile< Dietary guanidineacetic acid supplementation ameliorated meat quality and regulated muscle metabolism of broilers subjected to pre-slaughter transport stress by metabolomics analysis>, HPLC of Formula: 112-63-0, the main research area is guanidineacetic acid dietary supplementation broiler meat muscle metabolism metabolomics; Qiandongnan Xiaoxiang chicken; gas chromatography−mass spectrometry; guanidine acetic acid; muscle metabolomics; pre-slaughter transport stress.
Pre-slaughter transport stress could induce multiple comprehensive variations in physiol. and metabolic parameters of broilers. However, the entire metabolomics of pre-slaughter transport stress and supplementation of exogenous energy regulatory substances on broilers is still poorly understood. The metabolome characteristics of broilers subjected to 3 h pre-slaughter transport stress combined with 1,200 mg/kg guanidinoacetic acid (GAA1,200) supplementation were analyzed using gas chromatog.-mass spectrometry (GC-MS) in this study. The results showed that, compared to the control group (no transport), 3 h pre-slaughter transport stress (T3h) decreased creatine (Cr), phosphocreatine (PCr) and ATP (ATP), and increased ADP (ADP), adenosine monophosphate (AMP) and the ratio of AMP to ATP in pectoralis muscle (PM) of broilers by high performance liquid chromatog. (HPLC) anal. However, GAA1,200 supplementation reversed the neg. effects induced by 3 h pre-slaughter transport stress. Besides, GAA1,200 supplementation elevated mRNA expression of creatine transporter in PM. Our metabolomics approaches demonstrated that 38 and 48 significant metabolites were sep. identified between the control group and T3h group, and T3h group and 3 h pre-slaughter transport stress combined with GAA1,200 supplementation group using the standard of variable importance in the projection values >1 and P < 0.05. Among these, the metabolites involved in amino acid metabolism (alanine, glycine, serine, threonine, cysteine , methionine, phenylalanine, tyrosine, and tryptophan), oxidative stress (3-methylhistidine, 1-methylhistidine and glutathione), non-protein amino acid (citrulline) metabolism, and energy metabolism (Cr, PCr, sarcosine, and glycocyamine) were confirmed through pathway enrichment anal., which could be chosen as suitable candidate targets for further anal. of the effects of exogenous energy substances on broilers subjected to transport stress. Poultry Science published new progress about Amino acids Role: FFD (Food or Feed Use), BIOL (Biological Study), USES (Uses). 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, HPLC of Formula: 112-63-0.
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