Lee, Wonbae; Lee, Yong-Ill; Lee, Jeonghoon; Davis, Lloyd M.; Deininger, Prescott; Soper, Steven A. published the artcile< Cross-talk-free dual-color fluorescence cross-correlation spectroscopy for the study of enzyme activity>, Application of C19H34O2, the main research area is enzyme cross talk free fluorescence cross correlation spectroscopy FCCS.
We have developed an instrument for spectral cross-talk-free dual-color fluorescence cross-correlation spectroscopy (FCCS), which provides a readout modality for the study of enzyme activity in application areas such as high-throughput screening. Two spectrally distinct (∼250 nm) fluorophores, Cy3 and IRD800, were excited simultaneously using two different excitation sources: one poised at 532 nm and the other at 780 nm. The fluorescence information was processed on two different color channels monitored with single-photon avalanche diodes (SPADs) that could transduce events at the single-mol. level. The system provided no color cross-talk (cross-excitation and/or cross-emission) and/or fluorescence resonance energy transfer (FRET), significantly improving data quality. To provide evidence of cross-talk-free operation, the system was evaluated using bright microspheres (λabs = 532 nm, λem = 560 nm) and quantum dots (λabs = 532 nm, λem = 810 nm). Exptl. results indicated that no color leakage from the microspheres or quantum dots into inappropriate color channels was observed To demonstrate the utility of the system, the enzymic activity of APE1, which is responsible for nicking the phosphodiester backbone in DNA on the 5′ side of an apurinic/apyrimidinic site, was monitored by FCCS using a double-stranded DNA substrate dual labeled with Cy3 and IRD800. Activity of APE1 was also monitored in the presence of an inhibitor (7-nitroindole-2-carboxylic acid) of the enzyme using this cross-talk-free FCCS platform. In all cases, no spectral leakage from single-mol. events into inappropriate color channels was observed
Analytical Chemistry (Washington, DC, United States) published new progress about Avalanche photodiodes (SPAD, single-photon). 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Application of C19H34O2.
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Ester – Wikipedia,
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