Wang, Jun-lin; Hu, Ling-ling; Cai, Zeng-xuan; Li, Jin; Ren, Yi-ping published the artcile< Comparison and assessment of aflatoxins determination by ultra-pressure liquid chromatography>, Synthetic Route of 112-63-0, the main research area is UPLC trifluoroacetic acid aflatoxin purification food analysis contamination.
Objective: To compare and evaluate several methods for determination of aflatoxins by ultra- pressure liquid chromatog. (UPLC) combined with fluorescence detection (FLD). Methods: Samples were extracted by homogeneity (vortex) and ultrasound-assisted liquid-liquid extraction, and purified by immunoaffinity column and multifunctional column. Then the analytes were analyzed by UPLC-FLD with trifluoroacetic acid (TFA) pre-column derivation, photochem. post column derivation, iodine and bromine post column derivation, large volume flow cell and photochem. post column derivation tandem large volume flow cell. Results: The results showed each method was satisfying, and each method could fulfill the inspection requirements of common samples. Conclusion: The method of UPLC combined with fluorescence detector and large volume flow cell had the highest sensitivity among these methods. This method could not only raise the work efficiency, but also reduce a lot of poisonous and harmful organic solvent. Meanwhile, the method successfully fulfilled the min. limiting level requests according to the GB 2761-2011. Both acetonitnle aqueous solution and methanol aqueous solution could extract the aflatoxins from samples successfully. And the cleanup effect of immunoaffinity column achieved better results than that of multifunctional column.
Shipin Anquan Zhiliang Jiance Xuebao published new progress about Corn. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Synthetic Route of 112-63-0.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics