Huang, Danyun; Li, Zhijun; Chen, Yue; Fan, Yan; Yu, Tao published the artcile< Paeoniflorin reduces the inflammatory response of THP-1 cells by up-regulating microRNA-124 - Paeoniflorin reduces the inflammatory response of THP-1 cells through microRNA-124>, Reference of 112-63-0, the main research area is paeoniflorin THP cell microRNA inflammatory response; Apoptosis; Paeoniflorin; Proliferation; THP-1 cells; miRNA-124.
The activation of macrophages and the release of inflammatory cytokines are the main reasons for the progress of systemic lupus erythematosus (SLE). MicroRNA (miRNA)-124 is involved in the regulation of macrophages and is a key regulator of inflammation and immunity. To explore whether paeoniflorin (PF) regulates the biol. functions of macrophages depends on miR-124. RT-PCR, WB, ELISA, CCK-8 and flow cytometry were used to evaluate that PF regulated the biol. functions of THP-1 cells through miR-124. PF significantly inhibited the proliferation while promotes the apoptosis of THP-1 cells, and inhibited the release of IL-6, TNF-α and IL-1β in THP-1 cells. RT-PCR results shown that PF up-regulated the expression of miR-124 in THP-1 cells. Functional recovery experiments showed that compared with the LPS + mimic-NC group, LPS + miR-124 mimic significantly inhibited the proliferation and the release of IL-6, TNF-α and IL-1β, but promoted the apoptosis of THP-1 cells. In addition, compared with the LPS + PF + inhibitor-NC group, LPS + PF + miR-124 inhibitor significantly promoted the proliferation and the release of IL-6, TNF-α and IL-1β, but inhibited the apoptosis of THP-1 cells. By down-regulating miR-124, PF inhibits the proliferation and inflammation of THP-1 cells, and promotes the apoptosis of THP-1 cells.
Genes & Genomics published new progress about Apoptosis. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Reference of 112-63-0.
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