Chu, Kevin L.; Koley, Somnath; Jenkins, Lauren M.; Bailey, Sally R.; Kambhampati, Shrikaar; Foley, Kevin; Arp, Jennifer J.; Morley, Stewart A.; Czymmek, Kirk J.; Bates, Philip D.; Allen, Doug K. published the artcile< Metabolic flux analysis of the non-transitory starch tradeoff for lipid production in mature tobacco leaves>, Electric Literature of 112-63-0, the main research area is nontransitory starch lipid production tobacco leaf triacylglycerol; (13)C isotope Labeling; Acyl-ACPs; Carbon partitioning; Metabolic flux analysis; Nicotiana tabacum (tobacco); Starch-triacylglycerol tradeoff.
The metabolic plasticity of tobacco leaves has been demonstrated via the generation of transgenic plants that can accumulate over 30% dry weight as triacylglycerols. In investigating the changes in carbon partitioning in these high lipid-producing (HLP) leaves, foliar lipids accumulated stepwise over development. Interestingly, non-transient starch was observed to accumulate with plant age in WT but not HLP leaves, with a drop in foliar starch concurrent with an increase in lipid content. The metabolic carbon tradeoff between starch and lipid was studied using 13CO2-labeling experiments and isotopically nonstationary metabolic flux anal., not previously applied to the mature leaves of a crop. Fatty acid synthesis was investigated through assessment of acyl-acyl carrier proteins using a recently derived quantification method that was extended to accommodate isotopic labeling. Anal. of labeling patterns and flux modeling indicated the continued production of unlabeled starch, sucrose cycling, and a significant contribution of NADP-malic enzyme to plastidic pyruvate production for the production of lipids in HLP leaves, with the latter verified by enzyme activity assays. The results suggest an inherent capacity for a developmentally regulated carbon sink in tobacco leaves and may in part explain the uniquely successful leaf lipid engineering efforts in this crop.
Metabolic Engineering published new progress about Chloroplast. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Electric Literature of 112-63-0.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics