Sghaier, Randa; Nury, Thomas; Leoni, Valerio; Caccia, Claudio; Pais De Barros, Jean-Paul; Cherif, Ameur; Vejux, Anne; Moreau, Thibault; Limem, Khalifa; Samadi, Mohammad; Mackrill, John J.; Masmoudi, Ahmed Slaheddine; Lizard, Gerard; Zarrouk, Amira published the artcile< Dimethyl fumarate and monomethyl fumarate attenuate oxidative stress and mitochondrial alterations leading to oxiapoptophagy in 158N murine oligodendrocytes treated with 7β-hydroxycholesterol>, Category: esters-buliding-blocks, the main research area is oligodendrocyte oxiapoptophagy dimethyl monomethyl fumarate oxidative stress mitochondrial dysfunction; 158N cells; 7β-hydroxycholesterol; Apoptosis; Autophagy; Dimethyl fumarate; Lipid profile; Mitochondria; Monomethyl fumarate; Oxiapoptophagy; Oxidative stress; Peroxisome.
The cytoprotective effects of dimethylfumarate, used in the treatment of relapsing remitting multiple sclerosis and of MMF, its main metabolite, were evaluated on murine oligodendrocytes 158 N exposed to 7β-OHC (50μM, 24 h) with or without DMF or MMF (25μM). The activity of 7β-OHC in the presence or absence DMF or MMF was evaluated on several parameters: cell adhesion; plasma membrane integrity measured with PI, trypan blue and FDA assays; LDH activity; antioxidant enzyme activities ; generation of lipid peroxidation products (MDA, CDs) and CPs; ROS overproduction conducted with DHE and DHR123. Apoptosis and autophagy were characterized by staining with Hoechst 33,342, Giemsa and acridine orange, and with antibodies raised against caspase-3 and LC3. DMF and MMF attenuate 7β-OHC-induced cytotoxicity: cell growth inhibition; decreased cell viability; mitochondrial dysfunction (decrease of succinate dehydrogenase activity, loss of ΔΨm, increase of mitochondrial O·-2 production, alteration of the TCA cycle, and cardiolipins content); oxidative stress induction; changes in fatty acid and cholesterol metabolism; and cell death induction (caspase-3 cleavage, activation of LC3-I in LC3-II). Ultrastructural alterations of mitochondria and peroxisomes were prevented. These results demonstrate that DMF and MMF prevent major dysfunctions associated with neurodegenerative diseases: oxidative stress, mitochondrial dysfunction, apoptosis and autophagy.
Journal of Steroid Biochemistry and Molecular Biology published new progress about Antioxidants. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Category: esters-buliding-blocks.
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