Month: December 2022

Cao, Yang published the artcileAlterations in the gut microbiome and metabolic profile in rats acclimated to high environmental temperature, COA of Formula: C7H12O3, the main research area is animal metabolism acclimation environmental temperature gut microbiome taxonomy.

Heat acclimation (HA) is the best strategy to improve heat stress tolerance by inducing pos. physiol. adaptations. Evidence indicates that the gut microbiome plays a fundamental role in the development of HA, and modulation of gut microbiota can improve tolerance to heat exposure and decrease the risks of heat illness. In this study, for the first time, we applied 16S rRNA gene sequencing and untargeted liquid chromatog.-mass spectrometry (LC-MS) metabolomics to explore variations in the gut microbiome and faecal metabolic profiles in rats after HA. The gut microbiota of HA subjects exhibited higher diversity and richer microbes. HA altered the gut microbiota composition with significant increases in the genera Lactobacillus (a major probiotic) and Oscillospira alongside significant decreases in the genera Blautia and Allobaculum. The faecal metabolome was also significantly changed after HA, and among the 13 perturbed metabolites, (S)-AL 8810 and celastrol were increased. Moreover, the two increased genera were pos. correlated with the two upregulated metabolites and neg. correlated with the other 11 downregulated metabolites, while the correlations between the two decreased genera and the upregulated/downregulated metabolites were completely contrary. In summary, both the structure of the gut microbiome community and the faecal metabolome were improved after 28 days of HA. These findings provide novel insights regarding the improvement of the gut microbiome and its functions as a potential mechanism by which HA confers protection against heat stress.

Microbial Biotechnology published new progress about 16S rRNA Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 539-88-8 belongs to class esters-buliding-blocks, name is Ethyl 4-oxopentanoate, and the molecular formula is C7H12O3, COA of Formula: C7H12O3.

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Su, Chang published the artcileEffects of Saccharomycopsis fibuligera and Saccharomyces cerevisiae inoculation on small fermentation starters in Sichuan-style Xiaoqu liquor, Synthetic Route of 123-29-5, the main research area is Saccharomycopsis Saccharomyces xiaoqu liquor fermentation starter; Bioaugmentation inoculation; Correlation analysis; High-throughput sequencing; Traditional isolation; Xiaoqu liquor.

Xiaoqu liquor is a type of distilled spirit in China prepared on a small scale from a small solid starter culture. To improve the flavor profile of Xiaoqu liquor, we selected two functional yeast strains (Saccharomycopsis fibuligera and Saccharomyces cerevisiae) from Zaopei (fermented grain) of Baijiu liquor and used them for Xiaoqu liquor fermentation Compared with traditional Xiaoqu (Starter), bioaugmentation inoculation increased the glucoamylase and acidic protease activities and the ethanol synthesis rate, while decreasing the acidity of the Zaopei (fermented grains) in the early stage of fermentation By the end of the fermentation process, the alc. and ester content had also increased by 42.5% and 11.8%, resp., and that of aldehydes and ketones, and heterocyclic compounds decreased by 73.7% and 77.1%, resp. The dominant fungus during fermentation using the two types of starter cultures was S. cerevisiae, whereas the dominant bacteria was Pseudomonas, followed by Bacillus, Weissella, Lactobacillus, and Bacteroides. Principal component anal. of the bacterial community structure and flavor substances in the Zaopei produced using the two strains revealed that there were few differences between the two liquors and that inoculation with functional yeasts may not change the flavor substances in Xiaoqu liquor. However, correlation anal. showed that Escherichia Shigella, Terrisporobacter, Bacillus, Clostridium, and Prevotellaceae are the main microorganisms in the Xiaoqu liquor fermentation process.

Food Research International published new progress about 16S rRNA Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 123-29-5 belongs to class esters-buliding-blocks, name is Ethyl nonanoate, and the molecular formula is C11H22O2, Synthetic Route of 123-29-5.

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Su, Chang published the artcileEffects of Saccharomycopsis fibuligera and Saccharomyces cerevisiae inoculation on small fermentation starters in Sichuan-style Xiaoqu liquor, Name: Methyl octanoate, the main research area is Saccharomycopsis Saccharomyces xiaoqu liquor fermentation starter; Bioaugmentation inoculation; Correlation analysis; High-throughput sequencing; Traditional isolation; Xiaoqu liquor.

Xiaoqu liquor is a type of distilled spirit in China prepared on a small scale from a small solid starter culture. To improve the flavor profile of Xiaoqu liquor, we selected two functional yeast strains (Saccharomycopsis fibuligera and Saccharomyces cerevisiae) from Zaopei (fermented grain) of Baijiu liquor and used them for Xiaoqu liquor fermentation Compared with traditional Xiaoqu (Starter), bioaugmentation inoculation increased the glucoamylase and acidic protease activities and the ethanol synthesis rate, while decreasing the acidity of the Zaopei (fermented grains) in the early stage of fermentation By the end of the fermentation process, the alc. and ester content had also increased by 42.5% and 11.8%, resp., and that of aldehydes and ketones, and heterocyclic compounds decreased by 73.7% and 77.1%, resp. The dominant fungus during fermentation using the two types of starter cultures was S. cerevisiae, whereas the dominant bacteria was Pseudomonas, followed by Bacillus, Weissella, Lactobacillus, and Bacteroides. Principal component anal. of the bacterial community structure and flavor substances in the Zaopei produced using the two strains revealed that there were few differences between the two liquors and that inoculation with functional yeasts may not change the flavor substances in Xiaoqu liquor. However, correlation anal. showed that Escherichia Shigella, Terrisporobacter, Bacillus, Clostridium, and Prevotellaceae are the main microorganisms in the Xiaoqu liquor fermentation process.

Food Research International published new progress about 16S rRNA Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 111-11-5 belongs to class esters-buliding-blocks, name is Methyl octanoate, and the molecular formula is C9H18O2, Name: Methyl octanoate.

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Su, Chang published the artcileEffects of Saccharomycopsis fibuligera and Saccharomyces cerevisiae inoculation on small fermentation starters in Sichuan-style Xiaoqu liquor, Application of Ethyl pentadecanoate, the main research area is Saccharomycopsis Saccharomyces xiaoqu liquor fermentation starter; Bioaugmentation inoculation; Correlation analysis; High-throughput sequencing; Traditional isolation; Xiaoqu liquor.

Xiaoqu liquor is a type of distilled spirit in China prepared on a small scale from a small solid starter culture. To improve the flavor profile of Xiaoqu liquor, we selected two functional yeast strains (Saccharomycopsis fibuligera and Saccharomyces cerevisiae) from Zaopei (fermented grain) of Baijiu liquor and used them for Xiaoqu liquor fermentation Compared with traditional Xiaoqu (Starter), bioaugmentation inoculation increased the glucoamylase and acidic protease activities and the ethanol synthesis rate, while decreasing the acidity of the Zaopei (fermented grains) in the early stage of fermentation By the end of the fermentation process, the alc. and ester content had also increased by 42.5% and 11.8%, resp., and that of aldehydes and ketones, and heterocyclic compounds decreased by 73.7% and 77.1%, resp. The dominant fungus during fermentation using the two types of starter cultures was S. cerevisiae, whereas the dominant bacteria was Pseudomonas, followed by Bacillus, Weissella, Lactobacillus, and Bacteroides. Principal component anal. of the bacterial community structure and flavor substances in the Zaopei produced using the two strains revealed that there were few differences between the two liquors and that inoculation with functional yeasts may not change the flavor substances in Xiaoqu liquor. However, correlation anal. showed that Escherichia Shigella, Terrisporobacter, Bacillus, Clostridium, and Prevotellaceae are the main microorganisms in the Xiaoqu liquor fermentation process.

Food Research International published new progress about 16S rRNA Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 41114-00-5 belongs to class esters-buliding-blocks, name is Ethyl pentadecanoate, and the molecular formula is C17H34O2, Application of Ethyl pentadecanoate.

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Belleggia, Luca published the artcileIs there any still undisclosed biodiversity in Ciauscolo salami A new glance into the microbiota of an artisan production as revealed by high-throughput sequencing, Synthetic Route of 106-32-1, the main research area is Lactobacillus Leuconostoc Kurtzmaniella fermentation; Illumina sequencing; Kurtzmaniella zeylanoides; Lactobacillus algidus; Leuconostoc carnosum; Volatilome.

Ciauscolo is a fermented sausage with the Protected Geog. Indication (PGI) status. To disclose the microbial ecol. of a model Ciauscolo salami manufacture during its natural fermentation, viable counting, amplicon-based sequencing and real-time PCR were applied. The volatilome during fermentation was also characterized. The results allowed previously undetected species to be discovered. The core microbiota was composed by Lactobacillus algidus, Leuconostoc carnosum, Lactobacillus sakei, Debaryomyces hansenii, Glomus hyderabadensis, Tilletiopsis washingtonensis, and Kurtzmaniella zeylanoides. Salmonella spp. and Listeria monocytogenes were absent in all the samples; moreover, multiplex real-time PCR revealed the absence of the target genes bont/A, bont/B, bont/E, bont/F, and 4gyrB (CP), encoding botulinic toxins. Volatilome, deeply depending on microbiol. metabolism, was characterized by spices-derived components. Limonene, sabinene, α- and β-pinene, 3-carene, and α-thujene were the most represented monoterpene hydrocarbons, whereas β- and α-copaene were the most represented sesquiterpene hydrocarbons. Allyl Me sulfide and diallyl disulfide were the major aliphatic sulfur compounds, together with diallyl sulfide and allyl Me disulfide.

Meat Science published new progress about 16S rRNA Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 106-32-1 belongs to class esters-buliding-blocks, name is Ethyl octanoate, and the molecular formula is C10H20O2, Synthetic Route of 106-32-1.

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Liang, Jingjing published the artcileEffects of microbial community succession on volatile profiles and biogenic amine during sufu fermentation, Synthetic Route of 106-32-1, the main research area is microbial community succession volatile profile biogenic amine sufu fermentation.

In this study, we investigated microbial community succession and the metabolite changes during 84 days of sufu ripening (a traditional fermented soybean product). Fifty bacteria and ninety-nine fungi were identified. Cyanobacteria that was found in raw material tofu (72.79%) gradually decreased, and the bacterial communities involved in sufu fermentation were predominantly of genera Acinetobacter, Enterococcus and Streptococcus. For fungi, over the post fermentation stage (7-84 days), the top two genera includes Aspergillus and Monascus, accounting for approx. 95% of the whole genera. Seventy-two volatile flavours and seven biogenic amines were detected. And the results showed that sufu may be harmful mainly due to histamine intoxication. Redundancy anal. between microbiota and biogenic amine revealed that bacteria have a stronger effect on biogenic amine (except for cadaverine) than fungi, and histamine was pos. correlated with Kurthia. Spearman′s correlation (|ρ| > 0.7) anal. showed significant correlation between microbial profile and dominant flavor compounds, Macrococcus and Streptococcus were pos. correlated with 12 flavor compounds These findings help understand the fermentation mechanism and provide useful guidelines about the screening of strains that produce desirable flavours for the production of high quality and safe sufu.

LWT–Food Science and Technology published new progress about 16S rRNA Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 106-32-1 belongs to class esters-buliding-blocks, name is Ethyl octanoate, and the molecular formula is C10H20O2, Synthetic Route of 106-32-1.

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Liang, Jingjing published the artcileEffects of microbial community succession on volatile profiles and biogenic amine during sufu fermentation, Safety of Ethyl pentadecanoate, the main research area is microbial community succession volatile profile biogenic amine sufu fermentation.

In this study, we investigated microbial community succession and the metabolite changes during 84 days of sufu ripening (a traditional fermented soybean product). Fifty bacteria and ninety-nine fungi were identified. Cyanobacteria that was found in raw material tofu (72.79%) gradually decreased, and the bacterial communities involved in sufu fermentation were predominantly of genera Acinetobacter, Enterococcus and Streptococcus. For fungi, over the post fermentation stage (7-84 days), the top two genera includes Aspergillus and Monascus, accounting for approx. 95% of the whole genera. Seventy-two volatile flavours and seven biogenic amines were detected. And the results showed that sufu may be harmful mainly due to histamine intoxication. Redundancy anal. between microbiota and biogenic amine revealed that bacteria have a stronger effect on biogenic amine (except for cadaverine) than fungi, and histamine was pos. correlated with Kurthia. Spearman′s correlation (|ρ| > 0.7) anal. showed significant correlation between microbial profile and dominant flavor compounds, Macrococcus and Streptococcus were pos. correlated with 12 flavor compounds These findings help understand the fermentation mechanism and provide useful guidelines about the screening of strains that produce desirable flavours for the production of high quality and safe sufu.

LWT–Food Science and Technology published new progress about 16S rRNA Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 41114-00-5 belongs to class esters-buliding-blocks, name is Ethyl pentadecanoate, and the molecular formula is C17H34O2, Safety of Ethyl pentadecanoate.

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

De Pasquale, Ilaria published the artcileUse of autochthonous mesophilic lactic acid bacteria as starter cultures for making Pecorino Crotonese cheese: Effect on compositional, microbiological and biochemical attributes, Recommanded Product: Methyl octanoate, the main research area is Pecorino Crotonese cheese mesophilic lactic acid bacteria starter culture; Non-starter lactic acid bacteria; Pecorino Crotonese; Ripening; Starter cultures; Volatile components.

The use of selected autochthonous mesophilic lactic acid bacteria as starter cultures was investigated according to the traditional protocol for making Pecorino Crotonose (PC). Leuconostoc mesenteroides subsp. mesenteroides 2A, Lactobacillus casei 23C and Lactobacillus plantarum 18C (Autochthonous Starter, AS1) and Leuc. mesenteroides subsp. mesenteroides 2A, and L. casei 25D and 16A (AS2) were isolated and identified from aged ewes’ milk PC cheeses, selected based on several enzymic activities, and used as starter cultures. As shown by the in vitro kinetic of acidification, selected starter cultures had suitable capabilities to acidify. The manufacture of PC cheeses was carried out at an industrial plant scale. A control cheese (CC) was also made, using com. starters consisting of mesophilic and thermophilic species. Ripening lasted 105 days at 10 °C. A poly-phasic approach was used to compare cheeses during manufacture and ripening, mainly based on pyrosequencing of the 16S rRNA targeting DNA, proteolysis and volatile component analyses. Compared to CC, both autochthonous starter cultures slightly affected the gross chem. composition of PC cheese. The cell d. of thermophilic starters of CC progressively decreased throughout ripening. Plate count and RAPD-PCR showed that the cell number of autochthonous lactobacilli cultures of PC cheeses, made with AS1 and AS2, was almost constant throughout ripening and abundantly higher than that observed in CC. As shown by culture-independent anal., the OTUs found during ripening varied depending on the manufacture with or without autochthonous starter cultures. The major chem. differences among cheeses were the concentration of free amino acids and the synthesis of some key volatile components (e.g., 2-methyl-1-propanol, 2-methyl-1-butanol, isobutyric, isovaleric, and isocaproic acids). Compared to CC, the use of AS1 pos. affected the overall cheese quality.

Food Research International published new progress about 16S rRNA Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 111-11-5 belongs to class esters-buliding-blocks, name is Methyl octanoate, and the molecular formula is C9H18O2, Recommanded Product: Methyl octanoate.

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Gao, Jiangjing published the artcileConstructing simplified microbial consortia to improve the key flavor compounds during strong aroma-type Baijiu fermentation, Product Details of C10H20O2, the main research area is Baijiu fermentation flavor compound microbial consortia; Caproate; Ester formation; Simplified consortium; Top-down strategy.

The ester compounds play key roles in maintaining the sensory characteristics of alc. beverages. For strong aroma-type Baijiu fermentation, the volatile acids from pit mud microbes are key precursors for ester synthesis. However, the volatile acids can only be efficiently synthesized by the pit mud microbes in grains which attaches to pit mud. Elevating the ester contents in the upper layer’s fermented grains is vital to improve the quality of raw liquor. In this study, we applied top-down strategy and aim to simplify and obtain pit mud microbial consortia to efficiently produce caproate but not off-flavor compounds The simplified consortia with Caproiciproducens spp. as dominant species can use unsterilized fermentation water as sole substrate for caproate production, and stable caproate production was achieved by inoculating these simplified consortia in scaling-up fermentation The fermented broth was then applied to facilitate the fermentation of upper layer’s grains to prompt ester synthesis. Finally, the contents of variety esters such as Et caproate, Et pentanoate and Et octanoate were markedly increased. Together, this study demonstrates that constructing simplified microbial consortia containing key flavor-producing species is feasible to improve the flavor quality of spontaneously fermented foods.

International Journal of Food Microbiology published new progress about 16S rRNA Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 106-32-1 belongs to class esters-buliding-blocks, name is Ethyl octanoate, and the molecular formula is C10H20O2, Product Details of C10H20O2.

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Wu, Wei published the artcileExploring Core Microbiota Based on Characteristic Flavor Compounds in Different Fermentation Phases of Sufu, Synthetic Route of 106-32-1, the main research area is microbiota flavor compound fermentation phase sufu; bacterial community; co-occurring network; core microbiota; flavor components; sufu.

Sufu, a Chinese traditional fermented soybean product, has a characteristic foul smell but a pleasant taste. We determined the core functional microbiota and their metabolic mechanisms during sufu fermentation by examining relationships among bacteria, characteristic flavor compounds, and physicochem. factors. Flavor compounds in sufu were detected through headspace solid-phase microextraction coupled with gas chromatog.-mass spectrometry, and the microbial community structure was determined through high-throughput 16S rRNA sequencing. The results showed that the fermentation process of sufu could be divided into early and late stages. The early stage was critical for flavor development. Seven microbiota were screened based on their abundance, microbial relevance, and flavor production capacity. Five microbes were screened in the early stage: Pseudomonas, Tetragenococcus, Lysinibacillus, Pantoea, and Burkholderia-Caballeronia-Paraburkholderia. Three microbes were screened in the late stage: Exiguobacterium, Bacillus, and Pseudomonas. Their metabolic profiles were predicted. The results provided a reference for the selection of enriched bacterial genera in the fermentation process and controlling applicable process conditions to improve the flavor of sufu.

Molecules published new progress about 16S rRNA Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 106-32-1 belongs to class esters-buliding-blocks, name is Ethyl octanoate, and the molecular formula is C10H20O2, Synthetic Route of 106-32-1.

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics