Guo, Sixun published the artcileAnalysis of tizoxanide, active metabolite of nitazoxanide, in rat brain tissue and plasma by UHPLC-MS/MS, Computed Properties of 55981-09-4, the main research area is brain plasma tizoxanide nitazoxanide UHPLC MS; UHPLC-MS/MS; brain tissue; nitazoxanide; tizoxanide.
Tizoxanide, the active metabolite of nitazoxanide, has recently been reported as an effective agent for the treatment of glioma. As there had been no report about the anal. of tizoxanide in brain tissue, we established extraction and UHPLC-MS/MS methods to quantify tizoxanide in rat brain and plasma to evaluate the brain-to-plasma ratio of tizoxanide. The biol. samples were mainly prepared by acetonitrile and the separation was performed on a Waters XBridge BEH C18 column. The mobile phase was composed of water mixed with 10 mM ammonium formate (pH 3.0) and acetonitrile according a gradient volume Tizoxanide and topiramate (internal standard) were monitored utilizing neg. electron spray ionization in multiple reaction monitoring mode. The methods were validated to be precise and accurate within the dynamic range of 5-1000 ng/mL and 0.2-50 ng/g for plasma and brain tissue samples, resp. The lower limit of quantitation of the method was 0.2 ng/g, which was far more sensitive than all existing methods to quantify tizoxanide in biol. samples. Application performed on rats exhibited that the brain-to-plasma ratio of tizoxanide ranged from 3.16 to 26.86% in 1 h after administration of 10 mg/kg nitazoxanide.
Biomedical Chromatography published new progress about Brain. 55981-09-4 belongs to class esters-buliding-blocks, name is 2-((5-Nitrothiazol-2-yl)carbamoyl)phenyl acetate, and the molecular formula is C12H9N3O5S, Computed Properties of 55981-09-4.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics