Chapela, Sebastian P. published the artcileN-Acetyl cysteine improves cellular growth in respiratory-deficient yeast, Category: esters-buliding-blocks, the main research area is acetyl cysteine cellular growth respiratory deficient; Antioxidant effect; Reactive oxygen species (ROS); Yeast.
Reactive oxygen species (ROS) is a main factor that alters cellular physiol. and functionality. Many strategies are used in order to control excessive oxidative stress. One strategy includes the use of antioxidants like N-acetyl cysteine (NAC). The aim of this study was to compare the effect of this antioxidant on ROS production and cellular growth of a wild-type and a respiratory-deficient Saccharomyces cerevisiae strain. Using a simple system such as yeast allows oxidative stress investigations on which numerous factors are more manageable or circumscribed than in a higher organism. We grew cells in a complex medium and incubated them during 72 h. Later, cellular viability and ROS production was evaluated. ROS level was estimated by use of fluorescence signal with 2′,7′-dichlorofluorescein diacetate (DCFH-DA). As it is found in the present work, a reducing environment exerted by NAC presence during incubation of the cells allows a respiratory-deficient Saccharomyces cerevisiae strain to improve its cellular growth. It seems likely that the energy production or the phenotype which characterizes a deficient strain is incapable of palliating ROS growth inhibition while NAC helps to overcome this limitation.
Brazilian Journal of Microbiology published new progress about Antioxidants. 2044-85-1 belongs to class esters-buliding-blocks, name is 2′,7′-Dichloro-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl diacetate, and the molecular formula is C24H14Cl2O7, Category: esters-buliding-blocks.
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