Reiniers, Megan J. published the artcileAnalysis and optimization of conditions for the use of 2′,7′-dichlorofluorescein diacetate in cultured hepatocytes, HPLC of Formula: 2044-85-1, the main research area is hepatocyte oxidative stress 2 7 dichlorofluorescein diacetate optimization; anoxia and reoxygenation; cellular uptake and export; fluorogenic redox probe; hepatocytes; intravital fluorescence imaging; liver diseases; oxidative and nitrosative stress.
Numerous liver pathologies encompass oxidative stress as mol. basis of disease. The use of 2′,7′-dichlorodihydrofluorescein-diacetate (DCFH2-DA) as fluorogenic redox probe is problematic in liver cell lines because of membrane transport proteins that interfere with probe kinetics, among other reasons. The properties of DCFH2-DA were analyzed in hepatocytes (HepG2, HepaRG) to characterize methodol. issues that could hamper data interpretation and falsely skew conclusions. Experiments were focused on probe stability in relevant media, cellular probe uptake/retention/excretion, and basal oxidant formation and metabolism DCFH2-DA was used under optimized exptl. conditions to intravitally visualize and quantify oxidative stress in real-time in HepG2 cells subjected to anoxia/reoxygenation. The most important findings were that: (1) the non-fluorescent DCFH2-DA and the fluorescent DCF are rapidly taken up by hepatocytes, (2) DCF is poorly retained in hepatocytes, and (3) DCFH2 oxidation kinetics are cell type-specific. Furthermore, (4) DCF fluorescence intensity was pH-dependent at pH < 7 and (5) the stability of DCFH2-DA in cell culture medium relied on medium composition The use of DCFH2-DA to measure oxidative stress in cultured hepatocytes comes with methodol. and tech. challenges, which were characterized and solved. Optimized in vitro and intravital imaging protocols were formulated to help researchers conduct proper experiments and draw robust conclusions. Antioxidants published new progress about Antitumor agents. 2044-85-1 belongs to class esters-buliding-blocks, name is 2',7'-Dichloro-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate, and the molecular formula is C24H14Cl2O7, HPLC of Formula: 2044-85-1.
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