Gebner, Alec published the artcileCharacterization of extracellular vesicles from preconditioned human adipose-derived stromal/stem cells, Quality Control of 2044-85-1, the main research area is adipose derived mesenchymal stem cell hypoxia extracellular vesicle proteome; adipose-derived stromal/stem cells; extracellular vesicles; hypoxia; mesenchymal stromal/stem cells; proteomics; renal tubular epithelial cells.
Cell-free therapy using extracellular vesicles (EVs) from adipose-derived mesenchymal stromal/stem cells (ASCs) seems to be a safe and effective therapeutic option to support tissue and organ regeneration. The application of EVs requires particles with a maximum regenerative capability and hypoxic culture conditions as an in vitro preconditioning regimen has been shown to alter the mol. composition of released EVs. Nevertheless, the EV cargo after hypoxic preconditioning has not yet been comprehensively examined The aim of the present study was the characterization of EVs from hypoxic preconditioned ASCs. We investigated the EV proteome and their effects on renal tubular epithelial cells in vitro. While no effect of hypoxia was observed on the number of released EVs and their protein content, the cargo of the proteins was altered. Proteomic anal. showed 41 increased or decreased proteins, 11 in a statistically significant manner. Furthermore, the uptake of EVs in epithelial cells and a pos. effect on oxidative stress in vitro were observed In conclusion, culture of ASCs under hypoxic conditions was demonstrated to be a promising in vitro preconditioning regimen, which alters the protein cargo and increases the anti-oxidative potential of EVs. These properties may provide new potential therapeutic options for regenerative medicine.
International Journal of Molecular Sciences published new progress about Cell proliferation. 2044-85-1 belongs to class esters-buliding-blocks, name is 2′,7′-Dichloro-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl diacetate, and the molecular formula is C24H14Cl2O7, Quality Control of 2044-85-1.
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