Qiu, Shuai published the artcileChemoenzymatic catalysis of tert-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate by aldo-keto reductase coupled with composite Fe3O4 nanozyme scaffold, Quality Control of 539-88-8, the main research area is Exiguobacterium glucose dehydrogenase aldoketo reductase iron oxide nanoparticle.
Cofactor NADPH is widely used in enzymic redox reactions, however, its regeneration by glucose dehydrogenase (GDH) suffers from massive glucose consumption, costly wastewater treatment, and low atom efficiency. Herein, Fe3O4 nanoparticles supported on helical multi-walled carbon nanotubes (Fe3O4@HMWCNTs) was prepared and displayed comparable transfer hydrogenation activity to Exiguobacterium sibiricum GDH (EsGDH). We developed a chemoenzymic catalysis based on a combination of an aldo-keto reductase KmAKR with Fe3O4@HMWCNTs scaffold, which asym. synthesized optically pure tert-Bu 6-cyano-(3R,5R)-dihydroxyhexanoate ((3R,5R)-2, > 99.5% dep). Under the optimized conditions, 10 mM NADP+ was converted to NADPH by 5.0 mg Fe3O4@HMWCNTs, in a yield of > 80.0%. The combination of Fe3O4@HMWCNTs with KmAKRM7 completely reduced 10 mM tert-Bu 6-cyano-(5R)-hydroxy-3-oxohexanoate ((5R)-1) to (3R,5R)-2, in a conversion of > 99.0% and dep > 99.5%. Contrast to the previous developed biocatalytic approach with KmAKR and EsGDH, this efficient and compatible nanozyme-enzyme process required neither glucose/alcs. as co-substrate nor a glucose dehydrogenase/alc. dehydrogenase for NADPH regeneration.
Chemical Engineering Science published new progress about Alcohols, chiral Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 539-88-8 belongs to class esters-buliding-blocks, name is Ethyl 4-oxopentanoate, and the molecular formula is C7H12O3, Quality Control of 539-88-8.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics