Rai, Kamal published the artcileEnhanced anticoagulant activity of hirudin-i analogue co-expressed with arylsulfotransferase in periplasm of E. coli BL21(DE3), Quality Control of 6217-68-1, the publication is Journal of Biotechnology (2020), 107-112, database is CAplus and MEDLINE.
Hirudin, a blood anticoagulant, is the most potent natural thrombin inhibitor of leech origin. Its application is limited because it is difficult to obtain abundant natural hirudin directly from the leech. Although some bioengineering methods can significantly increase the production of hirudin, the reduced efficacy of recombinant hirudin (rH) remains a critical shortcoming. The lack of sulfation of tyrosine 63 in rH is an important cause of its inadequate performance. This article is the first report of periplasmic co-expression of an rH-I analog with arylsulfotransferase (ASST) in E. coli BL21(DE3). Co-expressed rH-I analog with sulfate donor substrate (p-nitrophenyl sulfate potassium) showed anticoagulant (rabbit and goat serum) activity twice more than rH-I analog expressed without ASST, indicating its potential periplasmic sulfation. Moreover, purified rH-I analog showed above 4.5 times higher anticoagulant activity compared to therapeutic anti-thrombotic heparin (HE). At the same time, pH-dependent differential solubility was employed to purify rH analogs from fermentation broth, which is a simple, fast and inexpensive purification technol., and can potentially be used for larger scale purification This will also greatly improve the application of rH in clin. treatment.
Journal of Biotechnology published new progress about 6217-68-1. 6217-68-1 belongs to esters-buliding-blocks, auxiliary class Salt,Nitro Compound,Sulfonate,Benzene, name is Potassium 4-nitrophenyl sulfate, and the molecular formula is C6H4KNO6S, Quality Control of 6217-68-1.
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