Gu, Lingwen published the artcileDimethyl itaconate protects against fungal keratitis by activating the Nrf2/HO-1 signaling pathway, Quality Control of 617-52-7, the publication is Immunology & Cell Biology (2020), 98(3), 229-241, database is CAplus and MEDLINE.
Di-Me itaconate (DI) is a membrane-permeable itaconate derivative with anti-inflammatory functions. However, the anti-inflammatory effect of DI has never been studied in fungal keratitis. In this study, we tested the protective effect of DI against fungal keratitis and assessed the role of Nrf2/HO-1 signaling in this process. Eyes of C57BL/6 (B6) mice were treated with 2 mm DI after infection with Aspergillus fumigatus. HCECs were pretreated with 0.25 mm DI and then incubated with A. fumigatus. Clin. scoring, slit-lamp photog., myeloperoxidase determination, flow cytometry and immunostaining were used to assess the disease response and treatment efficacy. PCR, Western blot and ELISA were used to assess the expression of IL-1β, chemokine (C-X-C motif) ligand 1, IL-6, IL-8, Nrf2 and HO-1. In addition, quantification of viable fungi, absorbance assays and fluorimetry were used to measure DI fungistatic activity. We observed that DI-treated eyes showed decreased clin. scores, fungal loads, PMN infiltration and cytokine expression, compared with phosphate-buffered saline-treated infected eyes. Moreover, DI treatment increased Nrf2 and HO-1 expression in corneas and nuclear Nrf2 accumulation in HCECs. Finally, DI treatment reduced the A. fumigatus absorbance and fungal mass. These data indicate that DI protects against fungal keratitis by limiting inflammation via the Nrf2/HO-1 signaling pathway and that DI inhibits the growth of A. fumigatus.
Immunology & Cell Biology published new progress about 617-52-7. 617-52-7 belongs to esters-buliding-blocks, auxiliary class Alkenyl,Aliphatic hydrocarbon chain,Ester, name is Dimethyl itaconate, and the molecular formula is C7H10O4, Quality Control of 617-52-7.
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