LC-MS-MS Simultaneous Determination of Paracetamol, Pseudoephedrine and Chlorpheniramine in Human Plasma: Application to a Pharmacokinetic Study was written by Liao, Qiongfeng;Xie, Zhiyong;Pan, Biyan;Zhu, Chenchen;Yao, Meicun;Xu, Xinjun;Wan, Jinzhi. And the article was included in Chromatographia in 2008.Quality Control of 4-Acetamidophenyl 2-acetoxybenzoate The following contents are mentioned in the article:
A liquid chromatog.-tandem mass spectrometry (LC-MS-MS) method was developed for the simultaneous determination of paracetamol, pseudoephedrine and chlorpheniramine in human plasma. Diphenhydramine was used as the internal standard Analytes were extracted from alkalized human plasma by liquid-liquid extraction (LLE) using Et acetate. After electrospray ionization pos. ion fragments were detected in the selected reaction monitoring (SRM) mode with a triple quadrupole tandem mass spectrometer. The method was linear in the concentration range of 20.0-10000.0 ng mL-1 for paracetamol, 1.0-500.0 ng mL-1 for pseudoephedrine and 0.1-50.0 ng mL-1 for chlorpheniramine. The intra- and inter-day precisions were below 14.5% and the bias was between -7.3 and +2.8% for all analytes. The validated LC-MS-MS method was applied to a pharmacokinetic study in which each healthy Chinese volunteer received a tablet containing 300 mg benorylate, 30 mg pseudoephedrine hydrochloride and 2 mg chlorpheniramine maleate. This is the first assay method described for the simultaneous determination of paracetamol, pseudoephedrine and chlorpheniramine in human plasma samples. This study involved multiple reactions and reactants, such as 4-Acetamidophenyl 2-acetoxybenzoate (cas: 5003-48-5Quality Control of 4-Acetamidophenyl 2-acetoxybenzoate).
4-Acetamidophenyl 2-acetoxybenzoate (cas: 5003-48-5) belongs to esters. Volatile esters with characteristic odours are used in synthetic flavours, perfumes, and cosmetics. Certain volatile esters are used as solvents for lacquers, paints, and varnishes. Esters contain a carbonyl center, which gives rise to 120° C–C–O and O–C–O angles. Unlike amides, esters are structurally flexible functional groups because rotation about the C–O–C bonds has a low barrier. Their flexibility and low polarity is manifested in their physical properties; they tend to be less rigid (lower melting point) and more volatile (lower boiling point) than the corresponding amides. Quality Control of 4-Acetamidophenyl 2-acetoxybenzoate
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