GC-MS Metabolite and Transcriptome Analyses Reveal the Differences of Volatile Synthesis and Gene Expression Profiling between Two Apple Varieties was written by Yang, Shunbo;Li, Dongmei;Li, Shanshan;Yang, Huijuan;Zhao, Zhengyang. And the article was included in International Journal of Molecular Sciences in 2022.Application of 112-14-1 The following contents are mentioned in the article:
Aroma is a key quality attribute of apples, making major contributions to com. value and consumer choice. However, the mechanism underlying mol. regulation of aroma formation genes and transcription factors remains poorly understood in apples. Here, we investigated the aroma volatile profiles of two apple varieties with distinctive flavors using headspace solid-phase microextraction (HS-SPME) combined with gas chromatog.-mass spectrometry (GC-MS). A total of 35 volatile compounds were identified in Granny Smith and Jonagold apples. Aldehydes were the most abundant volatiles contributing to the aroma in Granny Smith apple while esters were the dominant volatile compounds in Jonagold apple. In order to know more about the expression levels of aroma-related genes involved in the metabolic pathways, transcriptome sequencing of these two different apple varieties was conducted utilizing the Illumina platform. In total, 94 differentially expressed genes (DEGs) were found in the fatty acid metabolism, amino acid metabolism, the mevalonate pathway and phenylpropanoid pathway. Furthermore, compared to the Granny Smith apple, the expression of multiple genes and transcription factors were upregulated in the Jonagold apple, which might play important roles in the synthesis of aroma volatile compounds Our study contributes toward better understanding on the mol. mechanism of aroma synthesis in apples and provides a valuable reference for metabolic engineering and flavor improvement in the future. This study involved multiple reactions and reactants, such as Octyl acetate (cas: 112-14-1Application of 112-14-1).
Octyl acetate (cas: 112-14-1) belongs to esters. Esters typically have a pleasant smell; those of low molecular weight are commonly used as fragrances and are found in essential oils and pheromones. Cyclic esters are called lactones, regardless of whether they are derived from an organic or inorganic acid. One example of an organic lactone is γ-valerolactone.Application of 112-14-1
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics