Isolation, Purification and Characterization of Lipase from Microbacterium sp. and its Application in Biodiesel Production was written by Tripathi, Ritu;Singh, Jyoti;Bharti, Randhir kumar;Thakur, Indu Shekhar. And the article was included in Energy Procedia in 2014.Synthetic Route of C20H40O2 The following contents are mentioned in the article:
Presently the world is facing the problem of scarcity of fossil fuel as it is a non-renewable source of energy. Biodiesel can be an alternative energy source having the advantage of being renewable as well as environment friendly. Enzymes are better catalysts for the production biodiesel as enzymes are more stable and their production is more convenient and safer. Microalgae Scenedesmus sp. was used as a feedstock for biodiesel production Lipase producing bacteria was isolated from the pulp and paper mill and identified as Microbacterium sp. using 16S rDNA sequencing method. Lipase enzyme was purified by sequential methods of ammonium sulfate precipitation and Sephadex G-100 gel column chromatog. The mol. weight of purified enzyme was 40 kDa on SDS-PAGE. This purification procedure resulted in 2.1fold purification of lipase with a 20.8% final yield. The purified lipase exhibited maximal hydrolytic activity at a temperature of 50 0C and a pH of 8.5. The Km of lipase was 3.2 mM and the Vmax 50 渭mol/min/mg. Lipase activity was stimulated by Triton X-100 and SDS and inhibited by Tween 20 and Tween 80. Biodiesel was prepared through sodium hydroxide, potassium hydroxide and Lipase (Celite and charcoal bound as substrate) catalyzed transesterification process, which enabled a yield of 72.5%, 90% (95.1%, and 15.5%) resp. determined by gas chromatog./mass spectrometry (GC/MS) anal. This study involved multiple reactions and reactants, such as Methyl nonadecanoate (cas: 1731-94-8Synthetic Route of C20H40O2).
Methyl nonadecanoate (cas: 1731-94-8) belongs to esters. Carboxylic acid esters of low molecular weight are colourless, volatile liquids with pleasant odours, slightly soluble in water. Esters are more polar than ethers but less polar than alcohols. They participate in hydrogen bonds as hydrogen-bond acceptors, but cannot act as hydrogen-bond donors, unlike their parent alcohols. This ability to participate in hydrogen bonding confers some water-solubility.Synthetic Route of C20H40O2
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics