Porphyrin-Based Probe for Simultaneous Detection of Interface Acidity and Polarity during Lipid-Phase Transition of Vesicles was written by Majumder, Rini;Roy, Snigdha;Okamoto, Kentaro;Nagao, Satoshi;Matsuo, Takashi;Parui, Partha Pratim. And the article was included in Langmuir in 2020.COA of Formula: C16H22O11 The following contents are mentioned in the article:
Biochem. activities at a membrane interface are affected by local pH/polarity related to membrane lipid properties including lipid dynamics. pH and polarity at the interface are two highly interdependent parameters depending on various locations from the water-exposed outer-surface to the less-polar inner-surface. The optical response of common pH or polarity probes are affected by both the local pH and polarity; therefore, estimation of these values using two sep. probes localized at different interface depths can be erroneous. To estimate interface pH and polarity at an identical interface depth, we synthesized a glucose-pendant porphyrin (GPP) mol. for simultaneous pH and polarity detection by a single optical probe. pH-induced protonation equilibrium and polarity-dependent pi-pi stacking aggregation for GPP are exploited to measure pH and polarity changes at the 1,2-dimyristoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DMPG) membrane inter-face during DMPG phase transition. An NMR study confirmed that GPP is located at the interface Stern layer of DMPG large unilamellar vesicle (LUV). Using UV-vis absorption studies with an adapted anal. protocol, we estimated interface pH, or its deviation from the bulk phase value (螖pH), and the interface polarity simultaneously using the same spectra for sodium dodecyl sulfate (SDS) micelle and DMPG LUV. During temperature dependent gel to liquid-crystalline phase transition of DMPG, there was 鈭?.5 unit increase in 螖pH from approx. 鈭?.6 to 鈭?.1, with a small increase in interface dielec. constant from 鈭?0 to 63. A series of spectroscopic data indicate the utility of GPP for evaluation of local pH/polarity change during lipid phase transition of vesicles. This study involved multiple reactions and reactants, such as (2S,3R,4S,5R,6R)-6-(Acetoxymethyl)tetrahydro-2H-pyran-2,3,4,5-tetrayl tetraacetate (cas: 604-69-3COA of Formula: C16H22O11).
(2S,3R,4S,5R,6R)-6-(Acetoxymethyl)tetrahydro-2H-pyran-2,3,4,5-tetrayl tetraacetate (cas: 604-69-3) belongs to esters. Esters typically have a pleasant smell; those of low molecular weight are commonly used as fragrances and are found in essential oils and pheromones. Acyl chlorides and acid anhydrides alcoholysis is another way to produce esters. Acyl chlorides and acid anhydrides react with alcohols to produce esters. Anydrous conditions are recommended since both acyl chlorides and acid anhydrides react with water.COA of Formula: C16H22O11
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics