Wang, Zhenzhen; Jork, Nikolaus; Bittner, Tamara; Wang, Huanchen; Jessen, Henning J.; Shears, Stephen B. published an article in 2020, the title of the article was Rapid stimulation of cellular Pi uptake by the inositol pyrophosphate InsP8 induced by its photothermal release from lipid nanocarriers using a near infra-red light-emitting diode.Synthetic Route of 707-07-3 And the article contains the following content:
Inositol pyrophosphates (PP-InsPs), including diphospho-myo-inositol pentakisphosphate (5-InsP7) and bis-diphospho-myo-inositol tetrakisphosphate (1,5-InsP8), are highly polar, membrane-impermeant signaling mols. that control many homeostatic responses to metabolic and bioenergetic imbalance. To delineate their mol. activities, there is an increasing need for a toolbox of methodologies for real-time modulation of PP-InsP levels inside large populations of cultured cells. Here, we describe procedures to package PP-InsPs into thermosensitive phospholipid nanocapsules that are impregnated with a near infra-red photothermal dye; these liposomes are readily accumulated into cultured cells. The PP-InsPs remain trapped inside the liposomes until the cultures are illuminated with a near infra-red light-emitting diode (LED) which permeabilizes the liposomes to promote PP-InsP release. Addnl., so as to optimize these procedures, a novel stably fluorescent 5-InsP7 analog (i.e., 5-FAM-InsP7) was synthesized with the assistance of click-chem.; the delivery and deposition of the analog inside cells was monitored by flow cytometry and by confocal microscopy. We describe quant.-controlled PP-InsP release inside cells within 5 min of LED irradiation, without measurable effect upon cell integrity, using a collimated 22 mm beam that can irradiate up to 106 cultured cells. Finally, to interrogate the biol. value of these procedures, we delivered 1,5-InsP8 into HCT116 cells and showed it to dose-dependently stimulate the rate of [33P]-Pi uptake; these observations reveal a rheostatic range of concentrations over which 1,5-InsP8 is biol. functional in Pi homeostasis. The experimental process involved the reaction of (Trimethoxymethyl)benzene(cas: 707-07-3).Synthetic Route of 707-07-3
The Article related to inositol pyrophosphate cellular pi uptake photothermal release, Placeholder for records without volume info and other aspects.Synthetic Route of 707-07-3
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics