On January 31, 2019, Zhang, Ruijie K.; Chen, Kai; Huang, Xiongyi; Wohlschlager, Lena; Renata, Hans; Arnold, Frances H. published an article.Quality Control of 3-Acetyldihydrofuran-2(3H)-one The title of the article was Enzymatic assembly of carbon-carbon bonds via iron-catalysed sp3 C-H functionalization. And the article contained the following:
Although abundant in organic mols., carbon-hydrogen (C-H) bonds are typically considered unreactive and unavailable for chem. manipulation. Recent advances in C-H functionalization technol. have begun to transform this logic, while emphasizing the importance of and challenges associated with selective alkylation at a sp3 carbon. Here we describe iron-based catalysts for the enantio-, regio- and chemoselective intermol. alkylation of sp3 C-H bonds through carbene C-H insertion. The catalysts, derived from a cytochrome P 450 enzyme in which the native cysteine axial ligand has been substituted for serine (cytochrome P411), are fully genetically encoded and produced in bacteria, where they can be tuned by directed evolution for activity and selectivity. That these proteins activate iron, the most abundant transition metal, to perform this chem. provides a desirable alternative to noble-metal catalysts, which have dominated the field of C-H functionalization. The laboratory-evolved enzymes functionalize diverse substrates containing benzylic, allylic or α-amino C-H bonds with high turnover and excellent selectivity. Furthermore, they have enabled the development of concise routes to several natural products. The use of the native iron-haem cofactor of these enzymes to mediate sp3 C-H alkylation suggests that diverse haem proteins could serve as potential catalysts for this abiol. transformation, and will facilitate the development of new enzymic C-H functionalization reactions for applications in chem. and synthetic biol. The experimental process involved the reaction of 3-Acetyldihydrofuran-2(3H)-one(cas: 517-23-7).Quality Control of 3-Acetyldihydrofuran-2(3H)-one
The Article related to engineering cytochrome p411 heme iron substrate alkylation carbene insertion, Enzymes: Substrates-Cofactors-Inhibitors-Activators-Coenzymes-Products and other aspects.Quality Control of 3-Acetyldihydrofuran-2(3H)-one
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