On April 30, 2017, Eljezi, Teuta; Pinta, Pierre; Richard, Damien; Pinguet, Jeremy; Chezal, Jean-Michel; Chagnon, Marie-Christine; Sautou, Valerie; Grimandi, Gael; Moreau, Emmanuel published an article.Reference of Tris(2-ethylhexyl) benzene-1,2,4-tricarboxylate The title of the article was In vitro cytotoxic effects of DEHP-alternative plasticizers and their primary metabolites on a L929 cell line. And the article contained the following:
Phthalic acid esters have been widely used to improve the plasticity of PVC medical devices. They carry a high exposure risk for both humans and the environment in clin. situations. Our study focuses on the cytotoxicity of alternative plasticizers. Postulated primary metabolites were synthesized, not being com. available. Cytotoxicity assays were performed on L929 murine cells according to the ISO-EN 10993-5 standard design for the biocompatibility of medical devices. The tested concentrations of plasticizers (0.01, 0.05 and 0.1 mg/mL) covered the range likely to be found in biol. fluids coming into direct contact with the medical devices. DEHP, DINP and DINCH were cytotoxic at the highest concentration (0.1 mg/mL) for 7 days of exposure. Their corresponding metabolites were found to be more cytotoxic, for the same concentration By contrast, TOTM and its corresponding metabolite MOTM were not found to be cytotoxic. DEHA showed no cytotoxicity, but its corresponding monoester (MEHA) produced a cytotoxic effect at 0.05 mg/mL. In clin. situations, medical devices can release plasticizers, which can come into contact with patients. In vivo, the plasticizers are quickly transformed into primary metabolites. It is therefore important to measure the effects of both the plasticizers and their corresponding metabolites. Standard first-line cytotoxicity assays should be performed to ensure biocompatibility. The experimental process involved the reaction of Tris(2-ethylhexyl) benzene-1,2,4-tricarboxylate(cas: 3319-31-1).Reference of Tris(2-ethylhexyl) benzene-1,2,4-tricarboxylate
The Article related to fibroblast l929 cell line dehp alternative plasticizer metabolite cytotoxicity, cytotoxicity, dehp-alternative plasticizers, metabolite synthesis, phthalates, plasticizers and other aspects.Reference of Tris(2-ethylhexyl) benzene-1,2,4-tricarboxylate
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics