Balaratnam, Sumirtha; Rhodes, Curran; Bume, Desta Doro; Connelly, Colleen; Lai, Christopher C.; Kelley, James A.; Yazdani, Kamyar; Homan, Philip J.; Incarnato, Danny; Numata, Tomoyuki; Schneekloth, John S. Jr published the artcile< A chemical probe based on the PreQ1 metabolite enables transcriptome-wide mapping of binding sites>, Related Products of 112-63-0, the main research area is PreQ metabolite chem probe transcriptome binding site mapping.
The role of metabolite-responsive riboswitches in regulating gene expression in bacteria is well known and makes them useful systems for the study of RNA-small mol. interactions. Here, we study the PreQ1 riboswitch system, assessing sixteen diverse PreQ1-derived probes for their ability to selectively modify the class-I PreQ1 riboswitch aptamer covalently. For the most active probe (11), a diazirine-based photocrosslinking analog of PreQ1, X-ray crystallog. and gel-based competition assays demonstrated the mode of binding of the ligand to the aptamer, and functional assays demonstrated that the probe retains activity against the full riboswitch. Transcriptome-wide mapping using Chem-CLIP revealed a highly selective interaction between the bacterial aptamer and the probe. In addition, a small number of RNA targets in endogenous human transcripts were found to bind specifically to 11, providing evidence for candidate PreQ1 aptamers in human RNA. This work demonstrates a stark influence of linker chem. and structure on the ability of mols. to crosslink RNA, reveals that the PreQ1 aptamer/ligand pair are broadly useful for chem. biol. applications, and provides insights into how PreQ1, which is similar in structure to guanine, interacts with human RNAs.
Nature Communications published new progress about Aptamers. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Related Products of 112-63-0.
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