Winkler, Dirk F. H.; McGeer, Patrick L. published the artcile< Protein labeling and biotinylation of peptides during spot synthesis using biotin p-nitrophenyl ester (biotin-ONp)>, Application In Synthesis of 112-63-0, the main research area is protein labeling biotinylation peptide MALDI TOF mass spectrometry.
Biotin-labeled peptides are used for numerous biochem. and microbiol. applications. Due to the strong affinity of biotin to streptavidin, the detection of biotinylated mols. is very sensitive. A powerful technique for parallel synthesis and high-throughput screening of peptides is the spot synthesis. One example for the use of spot synthesis is the screening of biotinylated peptides synthesized on cellulose membranes, which is particularly favorable for the investigation of protease cleavage sites. Addnl., in combination with biotinylated protein samples, the spot technique can be used for investigations of peptide-protein and protein-protein interactions. Here, we present our results of the use biotin p-nitrophenyl ester (biotin-ONp) in spot synthesis and as a reagent for biotin-labeling of protein samples.
Proteomics published new progress about Biotinylation. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Application In Synthesis of 112-63-0.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics