Wentworth, Paul Jr.; Datta, Anita; Smith, Simon; Marshall, Ann; Partridge, Lynda J.; Blackburn, G. Michael published the artcile< Antibody Catalysis of BAc2 Aryl Carbamate Ester Hydrolysis: A Highly Disfavored Chemical Process>, Recommanded Product: (9Z,12Z)-Methyl octadeca-9,12-dienoate, the main research area is catalytic antibody aryl carbamate ester hydrolysis; monoclonal catalytic antibody aryl carbamate ester.
The alternative pathways for hydrolysis of an aryl carbamate ester have been differentiated by the use of a catalytic antibody. Monoclonal antibodies were generated against a tetrahedral phosphonamidate ester transition state analog (TSA) designed to lower the activation energy for the disfavored BAc2 process relative to that for the normal E1cB mechanism. Of the fifty antibodies elicited, DF8-D5 proved to be the best catalyst for hydrolysis of a p-nitrophenyl N-aryl carbamate, showing Michaelis-Menten kinetics with Km 120 μM, kcat 18 min-1, and was stoichiometrically inhibited by the TSA. Hammett anal. gave ρ = +0.53 for the DF8-D5 hydrolysis and ρ = 2.63 for the hydroxide mediated reaction of a range of p-substituted-Ph carbamates. For the p-nitrophenyl carbamate ester, DF8-D5 overcame a difference in activation energy between the uncatalyzed E1cB and BAc2 processes of 13 kcal mol-1, which is twice as great as any energy switch previously achieved by an antibody.
Journal of the American Chemical Society published new progress about Aromatic esters Role: BSU (Biological Study, Unclassified), BIOL (Biological Study) (carbamate). 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Recommanded Product: (9Z,12Z)-Methyl octadeca-9,12-dienoate.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics