Faulstich, Heinz; Zobeley, Suse; Bentrup, Ulla; Jockusch, Brigitte M. published the artcile< Biotinylphallotoxins: preparation and use as actin probes>, SDS of cas: 112-63-0, the main research area is electron microscopy actin filament biotinylphallotoxin; phalloidin derivative biotin preparation cytoskeleton.
The synthesis of four phalloidin derivatives conjugated with biotin is described. An aminomethyldithiolane derivative of ketophalloidin was used as a reactive starter compound, and biotin residues were coupled to this mol. either directly, separated by spacer chains comprised of one or two glycyl residues, or of a 12-atom long chain constructed from succinic acid and hexamethylenediamine. Although all products still displayed a high affinity for E-actin, as seen in competition experiments with [3H]demethylphalloidin, only the one with the longest spacer (BHPP) showed specific and high-affinity decoration of actin filaments in permeabilized cells, in conjunction with FITC-coupled avidin and fluorescence microscopy. Combined with gold-streptoavidin, BHPP decorated the actin filament system at the light and electron microscopic level faithfully and with satisfactory d. Actin filaments polymerized in vitro from purified protein were not as densely labeled as had been expected. However, in all these experiments the new phalloidin probe, when combined with avidin or streptoavidin, yielded clear and highly specific labeling of E-actin. Therefore, this system is useful to identify and localize actin unambiguously in microfilaments, independent of actin antibodies, and should facilitate double-label experiments on cystoskeletal components at the ultrastructural level.
Journal of Histochemistry and Cytochemistry published new progress about Avidins, conjugates Role: ANST (Analytical Study). 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, SDS of cas: 112-63-0.
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